HuB/D Polyclonal Antibody

    • Catalog No.:YT2261
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • HuB/D
      • Gene Name:
      • ELAVL2/ELAVL4
      • Protein Name:
      • ELAV-like protein 2/ELAV-like protein 4
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ELAV2/4. AA range:61-110
      • Specificity:
      • HuB/D Polyclonal Antibody detects endogenous levels of HuB/D protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ELAVL2;HUB;ELAV-like protein 2;ELAV-like neuronal protein 1;Hu-antigen B;HuB;Nervous system-specific RNA-binding protein Hel-N1;ELAVL4;HUD;PNEM;ELAV-like protein 4;Hu-antigen D;HuD;Paraneoplastic encephalomyelitis antigen HuD
      • Observed Band(KD):
      • 40kD
      • Background:
      • ELAV like RNA binding protein 2(ELAVL2) Homo sapiens The protein encoded by this gene is a neural-specific RNA-binding protein that is known to bind to several 3' UTRs, including its own and also that of FOS and ID. The encoded protein may recognize a GAAA motif in the RNA. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010],
      • Function:
      • function:Binds RNA. Seems to recognize a GAAA motif. Can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR.,similarity:Belongs to the RRM elav family.,similarity:Contains 3 RRM (RNA recognition motif) domains.,tissue specificity:Brain; neural-specific.,
      • Subcellular Location:
      • nucleoplasm,
      • Expression:
      • Brain; neural-specific.
      • Products Images
      • Western Blot analysis of Hela cells using HuB/D Polyclonal Antibody diluted at 1:1000
      • Immunofluorescence analysis of HepG2 cells, using ELAV2/4 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from Jurkat and HUVEC cells, using ELAV2/4 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from 293 cells using ELAV2/4 antibody.
      • Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).