HSP40 Polyclonal Antibody

    • Catalog No.:YT2254
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • HSP40
      • Fields:
      • >>Protein processing in endoplasmic reticulum;>>Influenza A
      • Gene Name:
      • DNAJB1
      • Protein Name:
      • DnaJ homolog subfamily B member 1
      • Human Gene Id:
      • 3337
      • Human Swiss Prot No:
      • P25685
      • Mouse Swiss Prot No:
      • Q9QYJ3
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human HSP40. AA range:271-320
      • Specificity:
      • HSP40 Polyclonal Antibody detects endogenous levels of HSP40 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • DNAJB1;DNAJ1;HDJ1;HSPF1;DnaJ homolog subfamily B member 1;DnaJ protein homolog 1;Heat shock 40 kDa protein 1;HSP40;Heat shock protein 40;Human DnaJ protein 1;hDj-1
      • Observed Band(KD):
      • 45kD
      • Background:
      • This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],
      • Function:
      • function:Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.,induction:By heat shock.,similarity:Contains 1 J domain.,subcellular location:Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.,subunit:Interacts with DNAJC3.,
      • Subcellular Location:
      • Cytoplasm . Nucleus . Nucleus, nucleolus . Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
      • Expression:
      • Brain,Heart,Lung,Placenta,
      • Products Images
      • Immunofluorescence analysis of Hela cell. 1,HSP40 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Caspase-8 Monoclonal Antibody(2G12)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western Blot analysis of various cells using HSP40 Polyclonal Antibody
      • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using HSP40 Antibody. The picture on the right is blocked with the synthesized peptide.