• Target：
• HM74

• Fields：
• >>cAMP signaling pathway

• Gene Name：
• HCAR2/HCAR3

• Protein Name：
• HM74A/HM74B

• Human Gene Id：
• 8843/338442

• Human Swiss Prot No：
• P49019/Q8TDS4

• Mouse Gene Id：
• 80885

• Rat Gene Id：
• 353250

• Rat Swiss Prot No：
• Q80Z39

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human GPR109. AA range:285-334

• Specificity：
• HM74 Polyclonal Antibody detects endogenous levels of HM74 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• HCAR3;GPR109B;HCA3;HM74B;NIACR2;Hydroxycarboxylic acid receptor 3;G-protein coupled receptor 109B;G-protein coupled receptor HM74;G-protein coupled receptor HM74B;Niacin receptor 2;Nicotinic acid receptor 2;HCAR2;GPR109A;HCA2;Hydroxycarboxylic acid receptor 2;

• Observed Band(KD)：
• 45kD

• Background：
• developmental stage:Expression in neutrophils occurs in the late terminal differentiation phase.,function:Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis.,miscellaneous:The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Expression largely restricted to adipose tissue and spleen. Expressed on mature neutrophils but not on immature neutrophils or eosinophils.,

• Function：
• developmental stage:Expression in neutrophils occurs in the late terminal differentiation phase.,function:Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis.,miscellaneous:The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-meth

• Subcellular Location：
• Cell membrane; Multi-pass membrane protein.

• Expression：
• Expression largely restricted to adipose tissue and spleen.

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