Hexim1 Polyclonal Antibody

    • Catalog No.:YT2129
    • Applications:WB,IHC-p,IF,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • HEXIM1
      • Protein Name:
      • Protein HEXIM1
      • Human Swiss Prot No:
      • O94992
      • Mouse Swiss Prot No:
      • Q8R409
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human HEXIM1. AA range:181-230
      • Specificity:
      • Hexim1 Polyclonal Antibody detects endogenous levels of Hexim1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • HEXIM1; CLP1; EDG1; HIS1; MAQ1; Protein HEXIM1; Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1
      • MolecularWeight(Da):
      • 40623
      • Observed Band(KD):
      • 40
      • Background:
      • hexamethylene bisacetamide inducible 1(HEXIM1) Homo sapiens Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008],
      • Function:
      • domain:The coiled-coil domain mediates oligomerization.,function:Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.,induction:Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). Down-regulated by estrogen.,miscellaneous:Inhibits Tat activity which is required for HIV-1 transcription.,similarity:Belongs to the HEXIM family.,subcellular location:Binds alpha-importin and is mostly nuclear.,subunit:Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, H
      • Subcellular Location:
      • nucleus,nucleoplasm,cytoplasm,
      • Expression:
      • Epithelium,Lung,Pericardium,Vascular smooth muscle,
      • Products Images
      • Western Blot analysis of RAT-HEART cells using Hexim1 Polyclonal Antibody diluted at 1:2000
      • Immunofluorescence analysis of HeLa cells, using HEXIM1 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from NIH/3T3 cells, using HEXIM1 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from HeLa cells using HEXIM1 antibody.