Granzyme H Polyclonal Antibody
- Catalog No.:YT2050
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Rat;Mouse;
- Target:
- Granzyme H
- Gene Name:
- GZMH
- Protein Name:
- Granzyme H
- Human Gene Id:
- 2999
- Human Swiss Prot No:
- P20718
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human GRAH. AA range:51-100
- Specificity:
- Granzyme H Polyclonal Antibody detects endogenous levels of Granzyme H protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- GZMH;CGL2;CTSGL2;Granzyme H;CCP-X;Cathepsin G-like 2;CTSGL2;Cytotoxic T-lymphocyte proteinase;Cytotoxic serine protease C;CSP-C
- Observed Band(KD):
- 23kD
- Background:
- This gene encodes a member of the peptidase S1 family of serine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a chymotrypsin-like protease. This protein is reported to be constitutively expressed in the NK (natural killer) cells of the immune system and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. This gene is present in a gene cluster with another member of the granzyme subfamily on chromosome 14. [provided by RefSeq, Nov 2015],
- Function:
- function:This enzyme is probably necessary for target cell lysis in cell-mediated immune responses.,similarity:Belongs to the peptidase S1 family.,similarity:Belongs to the peptidase S1 family. Granzyme subfamily.,similarity:Contains 1 peptidase S1 domain.,subcellular location:Cytoplasmic granules of cytolytic T-lymphocytes.,
- Subcellular Location:
- Cytolytic granule .
- Expression:
- Constitutively expressed in NK cells.
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- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of A549 cells, using GRAH Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from K562 cells, using GRAH Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HepG2 cells using NF1 antibody.
- Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200