GPR27 Polyclonal Antibody
- Catalog No.:YT2011
- Applications:WB;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- GPR27
- Gene Name:
- GPR27
- Protein Name:
- Probable G-protein coupled receptor 27
- Human Gene Id:
- 2850
- Human Swiss Prot No:
- Q9NS67
- Mouse Gene Id:
- 14761
- Mouse Swiss Prot No:
- O54897
- Rat Gene Id:
- 65275
- Rat Swiss Prot No:
- Q9JJH3
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human GPR27. AA range:181-230
- Specificity:
- GPR27 Polyclonal Antibody detects endogenous levels of GPR27 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- GPR27;SREB1;Probable G-protein coupled receptor 27;Super conserved receptor expressed in brain 1
- Observed Band(KD):
- 39kD
- Background:
- GPR27 is a member of the G protein-coupled receptors (GPCRs), a large family of receptors that have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli such as neurotransmitters, hormones, or light induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins, or G proteins.[supplied by OMIM, May 2010],
- Function:
- function:Orphan receptor. Possible candidate for amine-like G-protein coupled receptor.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Highly expressed as a 3.0 kb transcript in brain, ovary, testis, heart, prostate and peripheral Leukocytes. Lower levels in pancreas and small intestine. A 2.3 kb transcript was also found in peripheral Leukocytes. In brain regions, detected as a 3.0 kb transcript in all regions tested. Highest levels in the caudate nucleus, putamen, hippocampus and subthalamic nucleus. Lowest level in the cerebellum.,
- Subcellular Location:
- Cell membrane ; Multi-pass membrane protein .
- Expression:
- Highly expressed as a 3.0 kb transcript in brain, ovary, testis, heart, prostate and peripheral Leukocytes. Lower levels in pancreas and small intestine. A 2.3 kb transcript was also found in peripheral Leukocytes. In brain regions, detected as a 3.0 kb transcript in all regions tested. Highest levels in the caudate nucleus, putamen, hippocampus and subthalamic nucleus. Lowest level in the cerebellum.
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- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of A549 cells, using GPR27 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HUVEC cells, using GPR27 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using GPR27 antibody.