GPR18 Polyclonal Antibody
- Catalog No.:YT2005
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- GPR18
- Gene Name:
- GPR18
- Protein Name:
- N-arachidonyl glycine receptor
- Human Gene Id:
- 2841
- Human Swiss Prot No:
- Q14330
- Mouse Gene Id:
- 110168
- Mouse Swiss Prot No:
- Q8K1Z6
- Rat Gene Id:
- 679957
- Rat Swiss Prot No:
- A1A5S3
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human GPR18. AA range:191-240
- Specificity:
- GPR18 Polyclonal Antibody detects endogenous levels of GPR18 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- GPR18;GPCRW;N-arachidonyl glycine receptor;NAGly receptor;G-protein coupled receptor 18
- Observed Band(KD):
- 34kD
- Background:
- function:Receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. May contribute to regulation of the immune system.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells.,
- Function:
- function:Receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. May contribute to regulation of the immune system.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells.,
- Subcellular Location:
- Cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle membrane .
- Expression:
- Expressed in midpiece of spermatozoon (at protein level) (PubMed:27572937). Most abundant in testis and spleen (PubMed:16844083). Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells (PubMed:16844083).
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- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of HUVEC cells, using GPR18 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from 293 and Jurkat cells, using GPR18 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HUVECcells using GPR18 antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).