GPR18 Polyclonal Antibody

    • Catalog No.:YT2005
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • GPR18
      • Gene Name:
      • GPR18
      • Protein Name:
      • N-arachidonyl glycine receptor
      • Human Gene Id:
      • 2841
      • Human Swiss Prot No:
      • Q14330
      • Mouse Swiss Prot No:
      • Q8K1Z6
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human GPR18. AA range:191-240
      • Specificity:
      • GPR18 Polyclonal Antibody detects endogenous levels of GPR18 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • GPR18;GPCRW;N-arachidonyl glycine receptor;NAGly receptor;G-protein coupled receptor 18
      • Observed Band(KD):
      • 34kD
      • Background:
      • function:Receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. May contribute to regulation of the immune system.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells.,
      • Function:
      • function:Receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. May contribute to regulation of the immune system.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells.,
      • Subcellular Location:
      • Cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle membrane .
      • Expression:
      • Expressed in midpiece of spermatozoon (at protein level) (PubMed:27572937). Most abundant in testis and spleen (PubMed:16844083). Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells (PubMed:16844083).
      • Products Images
      • Immunofluorescence analysis of HUVEC cells, using GPR18 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from 293 and Jurkat cells, using GPR18 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from HUVECcells using GPR18 antibody.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).