• Target：
• Glucosidase IIα

• Fields：
• >>N-Glycan biosynthesis;>>Metabolic pathways;>>Protein processing in endoplasmic reticulum

• Gene Name：
• GANAB

• Protein Name：
• Neutral alpha-glucosidase AB

• Human Gene Id：
• 23193

• Human Swiss Prot No：
• Q14697

• Mouse Gene Id：
• 14376

• Mouse Swiss Prot No：
• Q8BHN3

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human GANAB. AA range:242-291

• Specificity：
• Glucosidase IIα Polyclonal Antibody detects endogenous levels of Glucosidase IIα protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• GANAB;G2AN;KIAA0088;Neutral alpha-glucosidase AB;Alpha-glucosidase 2;Glucosidase II subunit alpha

• Observed Band(KD)：
• 107kD

• Background：
• This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease. [provided by RefSeq, Jul 2016],

• Function：
• catalytic activity:Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans.,function:Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins.,pathway:Glycan metabolism; N-glycan metabolism.,similarity:Belongs to the glycosyl hydrolase 31 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC.,tissue specificity:Detected in placenta.,

• Subcellular Location：
• Endoplasmic reticulum . Golgi apparatus . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .

• Expression：
• Detected in placenta (PubMed:3881423). Isoform 1 and isoform 2 are expressed in the kidney and liver (PubMed:27259053).

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