GK2 Polyclonal Antibody

    • Catalog No.:YT1912
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Rat;Mouse;
      • Target:
      • GK2
      • Fields:
      • >>Glycerolipid metabolism;>>Metabolic pathways;>>PPAR signaling pathway
      • Gene Name:
      • GK2
      • Protein Name:
      • Glycerol kinase 2
      • Human Gene Id:
      • 2712
      • Human Swiss Prot No:
      • Q14410
      • Mouse Swiss Prot No:
      • Q9WU65
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human GK2. AA range:281-330
      • Specificity:
      • GK2 Polyclonal Antibody detects endogenous levels of GK2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • GK2;GKP2;GKTA;Glycerol kinase 2;GK 2;Glycerokinase 2;ATP:glycerol 3-phosphotransferase 2;Glycerol kinase; testis specific 2
      • Observed Band(KD):
      • 61kD
      • Background:
      • catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm the majority of the enzyme is bound to mitochondria.,
      • Function:
      • catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm the majority of the enzyme is bound to mitochondria.,
      • Subcellular Location:
      • Mitochondrion outer membrane ; Single-pass type IV membrane protein . Cytoplasm . In sperm the majority of the enzyme is bound to mitochondria. .
      • Expression:
      • Testis-specific (PubMed:33536340). Expressed in the midpiece of spermatozoa (PubMed:28852571).
      • Products Images
      • Western Blot analysis of various cells using GK2 Polyclonal Antibody
      • Western Blot analysis of Jurkat cells using GK2 Polyclonal Antibody
      • Western blot analysis of lysates from HeLa, 293, and Jurkat cells, using GK2 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).