• Target：
• MMP-9

• Fields：
• >>Endocrine resistance;>>IL-17 signaling pathway;>>TNF signaling pathway;>>Leukocyte transendothelial migration;>>Estrogen signaling pathway;>>Relaxin signaling pathway;>>Hepatitis B;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Proteoglycans in cancer;>>MicroRNAs in cancer;>>Prostate cancer;>>Bladder cancer;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis

• Gene Name：
• MMP9

• Protein Name：
• Matrix metalloproteinase-9

• Human Gene Id：
• 4318

• Human Swiss Prot No：
• P14780

• Mouse Swiss Prot No：
• P41245

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human MMP-9. AA range:651-700

• Specificity：
• MMP-9 Polyclonal Antibody detects endogenous levels of MMP-9 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MMP9;CLG4B;Matrix metalloproteinase-9;MMP-9;92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB

• Observed Band(KD)：
• 78kD

• Background：
• matrix metallopeptidase 9(MMP9) Homo sapiens Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:Cleavage of gelatin types I and V and collagen types IV and V.,cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 3 calcium ions per subunit.,disease:Defects in MMP9 may be a cause of susceptibility to lumbar disk herniation (LDH) [MIM:603932]. LDH is the predominant cause of low-back pain and unilateral leg pain.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,enzyme regulation:Inhibited by histatin-3 1/24 (histatin-5).,function:May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three qua

• Subcellular Location：
• Secreted, extracellular space, extracellular matrix .

• Expression：
• Detected in neutrophils (at protein level) (PubMed:7683678). Produced by normal alveolar macrophages and granulocytes.

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Nigericin Exerts Anticancer Effects on Human Colorectal Cancer Cells by Inhibiting Wnt/β-catenin Signaling Pathway. MOLECULAR CANCER THERAPEUTICS Mol Cancer Ther. 2018 May;17(5):952-965 WB,IF Human 1:300 SW620 cell，KM12 cell

LncRNA UCA1 promotes migration and invasion in pancreatic cancer cells via the Hippo pathway. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 2018 Mar 03 WB Human BxPC-3 cell, SW1990 cell,PaTu8988 cell, PANC-1 cell

MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells. Oncotarget Oncotarget. 2016 Mar 22; 7(12): 14476–14485 WB Human BxPC3 cell, PANC1 cell, SW1990 cell,PaTu8988 cell

Recruitment of monocytes and epigenetic silencing of intratumoral CYP7B1 primarily contribute to the accumulation of 27-hydroxycholesterol in breast cancer. American Journal of Cancer Research Am J Cancer Res. 2019; 9(10): 2194–2208 WB Human MCF-7 cell, MDA-MB-231 cell, THP-1 cell

CBX4 promotes the proliferation and metastasis via regulating BMI‐1 in lung cancer. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE J Cell Mol Med. 2020 Jan;24(1):618-631 WB Human 1:1000 NCI-H460 cell, A549 cell

MiR‐496 promotes migration and epithelial‐mesenchymal transition by targeting RASSF6 in colorectal cancer. JOURNAL OF CELLULAR PHYSIOLOGY J Cell Physiol. 2020 Feb;235(2):1469-1479 WB Human 1:1000 LoVo cell, SW480 cell

Inhibin B suppresses anoikis resistance and migration through the transforming growth factor‐β signaling pathway in nasopharyngeal carcinoma. CANCER SCIENCE Cancer Sci. 2018 Nov;109(11):3416-3427 WB Human HNE1 cell， 5-8F cell

Decreased level of miR-1301 promotes colorectal cancer progression via activation of STAT3 pathway. BIOLOGICAL CHEMISTRY Biol Chem. 2021 Jun;402(7):805-813 WB Human 1 : 1000 SW480 cell，LoVo cell

Phosphoglycerate mutase 1 knockdown inhibits prostate cancer cell growth, migration, and invasion. ASIAN JOURNAL OF ANDROLOGY Asian J Androl. 2018 Mar-Apr; 20(2): 178–183 WB Human 1:500 PC-3 cell,22Rv1 cell,DU145 cell, LNCap cell

Endothelin-1 Activates the Notch Signaling Pathway and Promotes Tumorigenesis in Giant Cell Tumor of the Spine. SPINE Spine. 2019 Sep;44(17):E1000-E1009 WB Human 1:500 giant cell tumor (GCT) tissue

Oleuropein inhibits the proliferation and invasion of glioma cells via suppression of the AKT signaling pathway. ONCOLOGY REPORTS 2016 Jul 28 WB Human U251 cell, A172 cell

Uridine-cytidine kinase 2 promotes metastasis of hepatocellular carcinoma cells via the Stat3 pathway. Cancer Management and Research Cancer Manag Res. 2018; 10: 6339–6355 WB Human 1:1000 Bel-7402 cell，Sk-hep-1 cell

Circular RNA circNFATC3 acts as a miR-9-5p sponge to promote cervical cancer development by upregulating SDC2. CELLULAR ONCOLOGY Cell Oncol. 2021 Feb;44(1):93-107 WB Human 1:800 HeLa cell,SiHa cell

Integration of exosomal miR-106a and mesothelial cells facilitates gastric cancer peritoneal dissemination. CELLULAR SIGNALLING Cell Signal. 2022 Mar;91:110230 WB Human 1:2000 HMrSV5 cell

miR-142 acts as a tumor suppressor in osteosarcoma cell lines by targeting Rac1. ONCOLOGY REPORTS Oncol Rep. 2015 Mar;33(3):1291-1299 WB Human Saos-2 cell, MG63 cell

Low-dose decitabine induces MAGE-A expression and inhibits invasion via suppression of NF-κB2 and MMP2 in Eca109 cells. BIOMEDICINE & PHARMACOTHERAPY 2014 Jul 18 WB Human 1:500 Eca109 cell

Insulin-like growth factor II mRNA binding protein 3 regulates proliferation, invasion and migration of neuroendocrine cancer cells. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2017; 10(10): 10269–10275 WB Mouse STC-1 cell

Protein arginine methyltransferase 1 coordinates the epithelial-mesenchymal transition/proliferation dichotomy in gastric cancer cells. EXPERIMENTAL CELL RESEARCH 2017 Oct 31 WB Human BGC823 cell, SGC7901 cell,AGS cell, MGC803 cell

YTH domain family 2 orchestrates epithelial-mesenchymal transition/proliferation dichotomy in pancreatic cancer cells. CELL CYCLE Cell Cycle. 2017;16(23):2259-2271 WB Human SW1990 cell, BxPC3 cell

Biological roles of human bone morphogenetic protein 9 in the bone microenvironment of human breast cancer MDA-MB-231 cells. American Journal of Translational Research Am J Transl Res. 2015; 7(9): 1660–1674 WB Human 1:1000 MDA-MB-231 cells,HS-5 cell

Long non‑coding RNA PVT1 promotes epithelial‑mesenchymal transition via the TGF‑β/Smad pathway in pancreatic cancer cells. ONCOLOGY REPORTS 2018 May 24 WB Human PaTu8988 cell, BxPc-3 cell

The elevated expression of TLR4 and MMP9 in human abdominal aortic aneurysm tissues and its implication. BMC Cardiovascular Disorders Bmc Cardiovasc Disor. 2021 Dec;21(1):1-11 IHC Human 1:200 Abdominal aortic aneurysm (AAA),non-aneurysmal contro tissues

CTGF enhances resistance to 5-FU-mediating cell apoptosis through FAK/MEK/ERK signal pathway in colorectal cancer. OncoTargets and Therapy Oncotargets Ther. 2016; 9: 7285–7295 WB Human PaTu8988 cell, SW1990 cell

Emodin suppresses the migration and invasion of melanoma cells. BIOLOGICAL & PHARMACEUTICAL BULLETIN 2021 Mar 17 WB Mouse,Human 1 : 500 B16F10 Melanoma Cells， A375 Melanoma Cells

Paeonol exerts potential activities to inhibit the growth, migration and invasion of human gastric cancer BGC823 cells via downregulating MMP‑2 and MMP‑9. Molecular Medicine Reports Mol Med Rep. 2017 Nov;16(5):7513-7519 WB Human 1:1000 BGC823 cell

Zhang, Hong, et al. "Therapeutic potential of bixin in PM2. 5 particles-induced lung injury in an Nrf2-dependent manner." Free Radical Biology and Medicine 126 (2018): 166-176.

Fang, Zhiqing, et al. "Nitidine chloride inhibits renal cancer cell metastasis via suppressing AKT signaling pathway." Food and chemical toxicology 60 (2013): 246-251.

Integrated transcriptome and proteome revealed that the declined expression of cell cycle-related genes associated with follicular atresia in geese BMC GENOMICS Xingyong Chen WB Geese follicles

HMGB1 released from dead tumor cells after insufficient radiofrequency ablation promotes progression of HCC residual tumor via ERK1/2 pathway INTERNATIONAL JOURNAL OF HYPERTHERMIA Baoming Luo WB Human HepG2 cell

LncRNA FAM83A-AS1 promotes epithelial–mesenchymal transition of pancreatic cancer cells via Hippo pathway. Min Xu WB Human BxPC-3 cell,PANC-1 cell,PANC-1 cell, PaTu8988 cell

Bufalin inhibits the proliferation of lung cancer cells by suppressing Hippo-YAP pathway. Yongchun Yu WB Human A549 cell

Rucaparib inhibits lung adenocarcinoma cell proliferation and migration via the SHCBP1/CDK1 pathway. FEBS Journal Rong Zhang WB Human 1 : 1000 A549 cell

Tangeretin attenuates bleomycin-induced pulmonary fibrosis by inhibiting epithelial-mesenchymal transition via the PI3K/Akt pathway. Frontiers in Pharmacology Pengfei Yi WB Mouse lung tissue

MBD3 promotes epithelial-mesenchymal transition in gastric cancer cells by upregulating ACTG1 via the PI3K/AKT pathway BIOLOGICAL PROCEDURES ONLINE Wang Huizhi WB Human MGC-803 cell,HGC-27 cell

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