• Target：
• GCSm-γ

• Fields：
• >>Cysteine and methionine metabolism;>>Glutathione metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors;>>Ferroptosis

• Gene Name：
• GCLM

• Protein Name：
• Glutamate--cysteine ligase regulatory subunit

• Human Gene Id：
• 2730

• Human Swiss Prot No：
• P48507

• Mouse Gene Id：
• 14630

• Mouse Swiss Prot No：
• O09172

• Rat Gene Id：
• 29739

• Rat Swiss Prot No：
• P48508

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human GCSm-gamma. AA range:42-91

• Specificity：
• GCSm-γ Polyclonal Antibody detects endogenous levels of GCSm-γ protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• GCLM;GLCLR;Glutamate--cysteine ligase regulatory subunit;GCS light chain;Gamma-ECS regulatory subunit;Gamma-glutamylcysteine synthetase regulatory subunit;Glutamate--cysteine ligase modifier subunit

• Observed Band(KD)：
• 31kD

• Background：
• Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015],

• Function：
• pathway:Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.,similarity:Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.,subunit:Heterodimer of a catalytic heavy chain and a regulatory light chain.,tissue specificity:In all tissues examined. Highest levels in skeletal muscle.,

• Subcellular Location：
• cytosol,glutamate-cysteine ligase complex,

• Expression：
• In all tissues examined. Highest levels in skeletal muscle.

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