• Target：
• Galectin-9

• Gene Name：
• LGALS9

• Protein Name：
• Galectin-9

• Human Gene Id：
• 3965

• Human Swiss Prot No：
• O00182

• Mouse Gene Id：
• 16859

• Mouse Swiss Prot No：
• O08573

• Rat Gene Id：
• 25476

• Rat Swiss Prot No：
• P97840

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human LEG9. AA range:51-100

• Specificity：
• Galectin-9 Polyclonal Antibody detects endogenous levels of Galectin-9 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• LGALS9;Galectin-9;Gal-9;Ecalectin;Tumor antigen HOM-HD-21

• Observed Band(KD)：
• 40kD

• Background：
• The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008],

• Function：
• alternative products:Additional isoforms seem to exist,domain:Contains two homologous but distinct carbohydrate-binding domains.,function:Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. The isoform Short acts as an eosinophil chemoattractant. Is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death.,online information:Galectin-9,similarity:Contains 2 galectin domains.,subcellular location:May also be secreted by a non-classical secretory pathway.,subunit:Monomer.,tissue specificity:Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin's disease tissue.,

• Subcellular Location：
• Cytoplasm . Nucleus . Secreted . May also be secreted by a non-classical secretory pathway (By similarity). Secreted by mesenchymal stromal cells upon IFNG stimulation (PubMed:23817958). .; [Isoform 2]: Secreted .; [Isoform 3]: Secreted .

• Expression：
• Peripheral blood leukocytes and lymphatic tissues. Expressed in lung, liver, breast and kidney with higher levels in tumor endothelial cells than normal endothelium (at protein level) (PubMed:24333696). Expressed in trophoblast cells in decidua and placenta in pregnancy (at protein level) (PubMed:23242525, PubMed:25578313). Isoform 2 is the most abundant isoform expressed in endothelial cells (PubMed:24333696). Upon endothelial cell activation isoform 2 expression decreases while expression of isoform 3 and isoform 5 increases (PubMed:24333696). Isoform 4 decreases in pathological pregnancy (PubMed:23242525).

microRNA-22 downregulation of galectin-9 influences lymphocyte apoptosis and tumor cell proliferation in liver cancer. ONCOLOGY REPORTS 2015 Jul 31 WB Human Lo2 cells ,HepG2 cell, SMMC7721 cell

miR‑455‑5p functions as a potential oncogene by targeting galectin‑9 in colon cancer. Oncology Letters 2017 Jan 17 WB Human 1:1000 HT29 cell

程庆, 王晓华, and 杨斌. "寻常型银屑病患者体内半乳糖凝集素家族蛋白表达研究." 皮肤性病诊疗学杂志 27.3 (2020): 141-146.

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs