Frizzled-8 Polyclonal Antibody
- Catalog No.:YT1787
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- Frizzled-8
- Fields:
- >>mTOR signaling pathway;>>Wnt signaling pathway;>>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells;>>Melanogenesis;>>Cushing syndrome;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Human papillomavirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Basal cell carcinoma;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer
- Gene Name:
- FZD8
- Protein Name:
- Frizzled-8
- Human Gene Id:
- 8325
- Human Swiss Prot No:
- Q9H461
- Mouse Gene Id:
- 14370
- Mouse Swiss Prot No:
- Q61091
- Rat Gene Id:
- 1.0091e+008
- Rat Swiss Prot No:
- Q498S8
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human FZD8. AA range:486-535
- Specificity:
- Frizzled-8 Polyclonal Antibody detects endogenous levels of Frizzled-8 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- FZD8;Frizzled-8;Fz-8;hFz8
- Observed Band(KD):
- 70kD
- Background:
- frizzled class receptor 8(FZD8) Homo sapiens This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq, Jul 2008],
- Function:
- domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,domain:The PDZ-binding motif mediates interaction with GOPC.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellul
- Subcellular Location:
- Membrane; Multi-pass membrane protein. Golgi apparatus . Cell membrane ; Multi-pass membrane protein . Colocalizes with GOPC at the Golgi apparatus. .
- Expression:
- Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from Jurkat cells, using FZD8 Antibody. The lane on the right is blocked with the synthesized peptide.