ERGIC-3 Polyclonal Antibody

    • Catalog No.:YT1620
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • ERGIC-3
      • Gene Name:
      • ERGIC3
      • Protein Name:
      • Endoplasmic reticulum-Golgi intermediate compartment protein 3
      • Human Swiss Prot No:
      • Q9Y282
      • Mouse Swiss Prot No:
      • Q9CQE7
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ERGI3. AA range:321-370
      • Specificity:
      • ERGIC-3 Polyclonal Antibody detects endogenous levels of ERGIC-3 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ERGIC3;C20orf47;ERV46;SDBCAG84;CGI-54;Endoplasmic reticulum-Golgi intermediate compartment protein 3;Serologically defined breast cancer antigen NY-BR-84
      • Observed Band(KD):
      • 43kD
      • Background:
      • function:Possible role in transport between endoplasmic reticulum and Golgi.,similarity:Belongs to the ERGIC family.,subcellular location:Cycles between the endoplasmic reticulum and the Golgi.,subunit:Interacts with ERGIC1/ERGIC32.,
      • Function:
      • function:Possible role in transport between endoplasmic reticulum and Golgi.,similarity:Belongs to the ERGIC family.,subcellular location:Cycles between the endoplasmic reticulum and the Golgi.,subunit:Interacts with ERGIC1/ERGIC32.,
      • Subcellular Location:
      • Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein . Golgi apparatus, cis-Golgi network membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cycles between the endoplasmic reticulum and the Golgi.
      • Expression:
      • Brain,Embryo,Fetal liver,Liver,Mammary gland,Skin,
      • Products Images
      • Immunofluorescence analysis of HepG2 cells, using ERGI3 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from LOVO and NIH/3T3 cells, using ERGI3 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).