• Target：
• eIF2α

• Fields：
• >>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Hepatitis C;>>Measles;>>Influenza A;>>Herpes simplex virus 1 infection;>>Lipid and atherosclerosis

• Gene Name：
• EIF2S1

• Protein Name：
• Eukaryotic translation initiation factor 2 subunit 1

• Human Gene Id：
• 1965

• Human Swiss Prot No：
• P05198

• Mouse Gene Id：
• 13665

• Mouse Swiss Prot No：
• Q6ZWX6

• Rat Gene Id：
• 54318

• Rat Swiss Prot No：
• P68101

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human eIF2 alpha. AA range:21-70

• Specificity：
• eIF2α Polyclonal Antibody detects endogenous levels of eIF2α protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• EIF2S1;EIF2A;Eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;eIF-2-alpha;eIF-2A;eIF-2alpha

• Observed Band(KD)：
• 38kD

• Background：
• The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010],

• Function：
• function:Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.,PTM:Substrate for at least 4 kinases: EIF2AK3/PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. In case of infection by vaccinia virus or rotavirus

• Subcellular Location：
• Cytoplasm, Stress granule . Colocalizes with NANOS3 in the stress granules. .

• Expression：
• B cells,Brain,Fibroblast,Placenta,

Huaier Induces Immunogenic Cell Death Via CircCLASP1/PKR/eIF2α Signaling Pathway in Triple Negative Breast Cancer WB Human /MDA-MB-231 cells

Enterovirus 71 Activates GADD34 via Precursor 3CD to Promote IRES-Mediated Viral Translation | Microbiology Spectrum Microbiol Spectr. 2022 Feb;:. WB Human

High carbohydrate diet induced endoplasmic reticulum stress and oxidative stress, promoted inflammation and apoptosis, impaired intestinal barrier of juvenile largemouth bass (Micropterus salmoides). FISH & SHELLFISH IMMUNOLOGY Fish Shellfish Immun. 2021 Dec;119:308 WB Fish 1:500 Intestinal

Overexpression of long non‐coding RNA NORAD promotes invasion and migration in malignant melanoma via regulating the MIR‐205‐EGLN2 pathway. Cancer Medicine Cancer Med-Us. 2019 Apr;8(4):1744-1754 WB Human A375 cell, WM35 cell

HRI of Epinephelus coioides is a critical factor in the grouper immune response to RGNNV infection. FISH & SHELLFISH IMMUNOLOGY 2019 Feb 10 WB Fish 1:1000 Grouper spleen (GS) cell

Isorhamnetin protects against bleomycin-induced pulmonary fibrosis by inhibiting endoplasmic reticulum stress and epithelial-mesenchymal transition. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 2019 Jan 01 WB Human,Mouse 1:1200 lung A549 cell,HBECs

Rce1 expression in renal cell carcinoma and its regulatory effect on 786-O cell apoptosis through endoplasmic reticulum stress. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2017 Mar 01 WB Human 1:2000 renal cancer tissues 786-O cell

Cadmium induces liver dysfunction and ferroptosis through the endoplasmic stress-ferritinophagy axis ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Jiacheng Wu WB Mouse

Shikonin potentiates therapeutic efficacy of oxaliplatin through reactive oxygen species-mediated intrinsic apoptosis and endoplasmic reticulum stress in oxaliplatin-resistant colorectal cancer cells DRUG DEVELOPMENT RESEARCH Yajie Zhang WB Human,Mouse HCT116R-xenograft OXA-resistant CRC cells (HCT116R)

Fusaric acid inhibits proliferation and induces apoptosis through triggering endoplasmic reticulum stress in MCF-7 human breast cancer cells. Yao Xiaoqiang WB Human 1:1000 MCF-7 cell

Dietary sodium acetate and sodium butyrate attenuate intestinal damage and improve lipid metabolism in juvenile largemouth bass (Micropterus salmoides) fed a high carbohydrate diet by reducing endoplasmic reticulum stress Animal Nutrition Liulan Zhao WB Fish 1:500 intestinal tissue

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