DNA pol β Polyclonal Antibody
- Catalog No.:YT1369
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- DNA pol β
- Fields:
- >>Base excision repair;>>Human T-cell leukemia virus 1 infection;>>Viral carcinogenesis
- Gene Name:
- POLB
- Protein Name:
- DNA polymerase beta
- Human Gene Id:
- 5423
- Human Swiss Prot No:
- P06746
- Mouse Gene Id:
- 18970
- Mouse Swiss Prot No:
- Q8K409
- Rat Gene Id:
- 29240
- Rat Swiss Prot No:
- P06766
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human DNA Polymerase beta. AA range:286-335
- Specificity:
- DNA pol β Polyclonal Antibody detects endogenous levels of DNA pol β protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- POLB;DNA polymerase beta
- Observed Band(KD):
- 38kD
- Background:
- The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011],
- Function:
- catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Binds 2 magnesium ions per subunit.,domain:Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.,function:Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.,PTM:Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.,similarity:Belongs to the DNA polymerase type-X family.,subunit:Monomer.,
- Subcellular Location:
- Nucleus. Cytoplasm. Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.
- Expression:
- Skin,Testis,
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using DNA pol β Polyclonal Antibody
- Western Blot analysis of K562 cells using DNA pol β Polyclonal Antibody
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DNA Polymerase beta Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from NIH/3T3 cells, using DNA Polymerase beta Antibody. The lane on the right is blocked with the synthesized peptide.