• Target：
• DNA Ligase I

• Fields：
• >>DNA replication;>>Base excision repair;>>Nucleotide excision repair;>>Mismatch repair

• Gene Name：
• LIG1

• Protein Name：
• DNA ligase 1

• Human Gene Id：
• 3978

• Human Swiss Prot No：
• P18858

• Mouse Swiss Prot No：
• P37913

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human DNL1. AA range:111-160

• Specificity：
• DNA Ligase I Polyclonal Antibody detects endogenous levels of DNA Ligase I protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• LIG1;DNA ligase 1;DNA ligase I;Polydeoxyribonucleotide synthase [ATP] 1

• Observed Band(KD)：
• 133kD

• Background：
• This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],

• Function：
• catalytic activity:ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).,cofactor:Magnesium.,disease:Defects in LIG1 seem to cause immunodeficiencies and cellular hypersensitivity to DNA-damaging agents.,function:DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.,online information:DNA ligase entry,online information:LIG1 mutation db,similarity:Belongs to the ATP-dependent DNA ligase family.,

• Subcellular Location：
• Nucleus.

• Expression：
• Brain,Epithelium,Eye,PCR rescued clones,T lymphoblast,

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