DHODH Polyclonal Antibody
- Catalog No.:YT1345
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Rat;Mouse;
- Target:
- DHODH
- Fields:
- >>Pyrimidine metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors
- Gene Name:
- DHODH
- Protein Name:
- Dihydroorotate dehydrogenase
- Human Gene Id:
- 1723
- Human Swiss Prot No:
- Q02127
- Mouse Swiss Prot No:
- O35435
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human DHODH. AA range:211-260
- Specificity:
- DHODH Polyclonal Antibody detects endogenous levels of DHODH protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- DHODH;Dihydroorotate dehydrogenase;quinone); mitochondrial;DHOdehase;Dihydroorotate oxidase
- Observed Band(KD):
- 48kD
- Background:
- The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008],
- Function:
- catalytic activity:(S)-dihydroorotate + O(2) = orotate + H(2)O(2).,cofactor:Binds 1 FAD per subunit.,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6.,similarity:Belongs to the dihydroorotate dehydrogenase family.,
- Subcellular Location:
- Mitochondrion inner membrane ; Single-pass membrane protein .
- Expression:
- Skin,Testis,
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- Antibody-FAQs
- Products Images
- Western Blot analysis of Jurkat cells using DHODH Polyclonal Antibody
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DHODH Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from Jurkat cells, using DHODH Antibody. The lane on the right is blocked with the synthesized peptide.