• Target：
• COL25A1

• Fields：
• >>Protein digestion and absorption

• Gene Name：
• COL25A1

• Protein Name：
• Collagen alpha-1(XXV) chain

• Human Gene Id：
• 84570

• Human Swiss Prot No：
• Q9BXS0

• Mouse Gene Id：
• 77018

• Mouse Swiss Prot No：
• Q99MQ5

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Collagen XXV alpha1. AA range:101-150

• Specificity：
• COL25A1 Polyclonal Antibody detects endogenous levels of COL25A1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• COL25A1;Collagen alpha-1(XXV) chain;Alzheimer disease amyloid-associated protein;AMY;CLAC-P

• Observed Band(KD)：
• 64kD

• Background：
• This gene encodes a brain-specific membrane associated collagen. A product of proteolytic processing of the encoded protein, CLAC (collagenous Alzheimer amyloid plaque component), binds to amyloid beta-peptides found in Alzheimer amyloid plaques but CLAC inhibits rather than facilitates amyloid fibril elongation (PMID: 16300410). A study of over-expression of this collagen in mice, however, found changes in pathology and behavior suggesting that the encoded protein may promote amyloid plaque formation (PMID: 19548013). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011],

• Function：
• caution:The pyrrolidone carboxylic acid reported in PubMed:11927537 probably formed artifactually from Glu-113 during the extraction procedure in 70% formic acid. In PubMed:15522881, the protein was found to have unblocked Glu at the N-terminus.,function:Inhibits fibrillization of beta amyloid peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin.,PTM:Glycosylated.,PTM:Hydroxylated on 11% of proline residues and 49% of lysine residues.,PTM:Undergoes proteolytic cleavage by furin protease to yield the soluble collagen-like Alzheimer amyloid plaque component.,similarity:Contains 7 collagen-like domains.,subcellular location:After proteolytic cleavage, CLAC is secreted.,subunit:Forms homodimers and homotrimers. Binds to the fibrillized forms of beta amyloid peptide 40 (beta-APP40) and beta amyloid peptide 42 (b

• Subcellular Location：
• Membrane ; Single-pass type II membrane protein . After proteolytic cleavage, CLAC is secreted.

• Expression：
• Expressed predominantly in brain. Deposited preferentially in primitive or neuritic amyloid plaques which are typical of Alzheimer disease.

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