CLIP3 Polyclonal Antibody
- Catalog No.:YT0969
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- CLIP3
- Gene Name:
- CLIP3
- Protein Name:
- CAP-Gly domain-containing linker protein 3
- Human Gene Id:
- 25999
- Human Swiss Prot No:
- Q96DZ5
- Mouse Gene Id:
- 76686
- Mouse Swiss Prot No:
- B9EHT4
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human CLIP3. AA range:361-410
- Specificity:
- CLIP3 Polyclonal Antibody detects endogenous levels of CLIP3 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- CLIP3;CLIPR59;CAP-Gly domain-containing linker protein 3;Cytoplasmic linker protein 170-related 59 kDa protein;CLIP-170-related 59 kDa protein;CLIPR-59
- Observed Band(KD):
- 60kD
- Background:
- This gene encodes a member of the cytoplasmic linker protein 170 family. Members of this protein family contain a cytoskeleton-associated protein glycine-rich domain and mediate the interaction of microtubules with cellular organelles. The encoded protein plays a role in T cell apoptosis by facilitating the association of tubulin and the lipid raft ganglioside GD3. The encoded protein also functions as a scaffold protein mediating membrane localization of phosphorylated protein kinase B. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010],
- Function:
- domain:Microtubule association is inhibited by the ANK repeats and the Golgi localization region (GoLD).,function:Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics.,miscellaneous:The N-terminal half is dispensable for proper Golgi targeting, whereas the GoLD region is required.,similarity:Contains 2 CAP-Gly domains.,similarity:Contains 3 ANK repeats.,subcellular location:Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae.,subunit:Homodimer.,
- Subcellular Location:
- Cell membrane ; Lipid-anchor . Cytoplasm . Golgi apparatus, Golgi stack . Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae.
- Expression:
- Brain,Skin,Uterus,
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using CLIP3 Polyclonal Antibody
- Immunofluorescence analysis of A549 cells, using CLIP3 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from A549 cells, using CLIP3 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).