CLIP3 Polyclonal Antibody

    • Catalog No.:YT0969
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • CLIP3
      • Gene Name:
      • CLIP3
      • Protein Name:
      • CAP-Gly domain-containing linker protein 3
      • Human Swiss Prot No:
      • Q96DZ5
      • Mouse Swiss Prot No:
      • B9EHT4
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human CLIP3. AA range:361-410
      • Specificity:
      • CLIP3 Polyclonal Antibody detects endogenous levels of CLIP3 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • CLIP3;CLIPR59;CAP-Gly domain-containing linker protein 3;Cytoplasmic linker protein 170-related 59 kDa protein;CLIP-170-related 59 kDa protein;CLIPR-59
      • Observed Band(KD):
      • 60kD
      • Background:
      • This gene encodes a member of the cytoplasmic linker protein 170 family. Members of this protein family contain a cytoskeleton-associated protein glycine-rich domain and mediate the interaction of microtubules with cellular organelles. The encoded protein plays a role in T cell apoptosis by facilitating the association of tubulin and the lipid raft ganglioside GD3. The encoded protein also functions as a scaffold protein mediating membrane localization of phosphorylated protein kinase B. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010],
      • Function:
      • domain:Microtubule association is inhibited by the ANK repeats and the Golgi localization region (GoLD).,function:Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics.,miscellaneous:The N-terminal half is dispensable for proper Golgi targeting, whereas the GoLD region is required.,similarity:Contains 2 CAP-Gly domains.,similarity:Contains 3 ANK repeats.,subcellular location:Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae.,subunit:Homodimer.,
      • Subcellular Location:
      • Cell membrane ; Lipid-anchor . Cytoplasm . Golgi apparatus, Golgi stack . Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae.
      • Expression:
      • Brain,Skin,Uterus,
      • Products Images
      • Western Blot analysis of various cells using CLIP3 Polyclonal Antibody
      • Immunofluorescence analysis of A549 cells, using CLIP3 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from A549 cells, using CLIP3 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).