• Target：
• CLIC4

• Gene Name：
• CLIC4

• Protein Name：
• Chloride intracellular channel protein 4

• Human Gene Id：
• 25932

• Human Swiss Prot No：
• Q9Y696

• Mouse Gene Id：
• 29876

• Mouse Swiss Prot No：
• Q9QYB1

• Rat Gene Id：
• 83718

• Rat Swiss Prot No：
• Q9Z0W7

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human CLIC4. AA range:1-50

• Specificity：
• CLIC4 Polyclonal Antibody detects endogenous levels of CLIC4 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CLIC4;Chloride intracellular channel protein 4;Intracellular chloride ion channel protein p64H1

• Observed Band(KD)：
• 29kD

• Background：
• chloride intracellular channel 4(CLIC4) Homo sapiens Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008],

• Function：
• domain:Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.,function:Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. May play a role in angiogenesis.,induction:Up-regulated by calcium ions in differentiating keratinocytes.,similarity:Belongs to the chloride channel CLIC family.,similarity:Contains 1 GST C-terminal domain.,subcellular location:Exists both as soluble cytoplasmic protein and as membrane protein with probably a single

• Subcellular Location：
• Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane ; Single-pass membrane protein . Nucleus matrix. Cell membrane ; Single-pass membrane protein . Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.

• Expression：
• Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.

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