• Target：
• CHSY2

• Fields：
• >>Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate;>>Metabolic pathways

• Gene Name：
• CHPF

• Protein Name：
• Chondroitin sulfate synthase 2

• Human Gene Id：
• 79586

• Human Swiss Prot No：
• Q8IZ52

• Mouse Gene Id：
• 74241

• Mouse Swiss Prot No：
• Q6IQX7

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human CHSY2. AA range:631-680

• Specificity：
• CHSY2 Polyclonal Antibody detects endogenous levels of CHSY2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CHPF;CSS2;Chondroitin sulfate synthase 2;Chondroitin glucuronyltransferase 2;Chondroitin-polymerizing factor;ChPF;Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II;N-acetylgalactosaminyl-pro

• Observed Band(KD)：
• 85kD

• Background：
• catalytic activity:UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.,catalytic activity:UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan.,cofactor:Divalent cations. Highest activities are measured with manganese. Can also utilize cobalt.,function:Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer.,online information:GlycoGene database,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the chondroitin N-acetylgalactosaminyltransferase family.,subunit:Binds CHSY1.,tissue specificity:Ubiquitous. Highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, small intestine and placenta.,

• Function：
• catalytic activity:UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.,catalytic activity:UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan.,cofactor:Divalent cations. Highest activities are measured with manganese. Can also utilize cobalt.,function:Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer.,online information:GlycoGene database,PTM:Phosphorylated upon DNA damage, p

• Subcellular Location：
• [Isoform 1]: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein . Cytoplasm, cytosol .; [Isoform 3]: Cytoplasm, cytosol . Mitochondrion .; [Isoform 2]: Mitochondrion matrix .

• Expression：
• Ubiquitous. Highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, spleen and placenta. ; [Isoform 2]: Expressed in brain, spleen, ovary, testis, lung and peripheral mononuclear cells. ; [Isoform 3]: Also ubiquitous.

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