• Target：
• Cdk2/Cdc2

• Fields：
• >>FoxO signaling pathway;>>Cell cycle;>>Oocyte meiosis;>>p53 signaling pathway;>>PI3K-Akt signaling pathway;>>Cellular senescence;>>Progesterone-mediated oocyte maturation;>>Cushing syndrome;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Viral carcinogenesis;>>Prostate cancer;>>Small cell lung cancer;>>Gastric cancer

• Gene Name：
• CDK2

• Protein Name：
• Cyclin-dependent kinase 2

• Human Gene Id：
• 1017

• Human Swiss Prot No：
• P24941

• Mouse Gene Id：
• 12566

• Mouse Swiss Prot No：
• P97377

• Rat Swiss Prot No：
• Q63699

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human CDK2. AA range:231-280

• Specificity：
• Cdk2 Polyclonal Antibody detects endogenous levels of Cdk2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CDK2;CDKN2;Cyclin-dependent kinase 2;Cell division protein kinase 2;p33 protein kinase

• Observed Band(KD)：
• 32kD

• Background：
• cyclin dependent kinase 2(CDK2) Homo sapiens This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-160 activates it.,function:Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Found in a complex with both SPDYA and CDKN1B/KIP1.,

• Subcellular Location：
• Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).

• Expression：
• Epithelium,Lung,Placenta,

Long noncoding RNA LINC01510 promotes the growth of colorectal cancer cells by modulating MET expression. Cancer Cell International Cancer Cell Int. 2018 Dec;18(1):1-12 WB Human LoVo cell, SW620 cell

Sirtuin 7 promotes non‑small cell lung cancer progression by facilitating G1/S phase and epithelial‑mesenchymal transition and activating AKT and ERK1/2 signaling. ONCOLOGY REPORTS Oncol Rep. 2020 Sep;44(3):959-972 WB,IHC Human 1 : 1000,1:100 Lung cancer TMA section A549 cell, H292 cell

Exosome-mediated transfer of MIF confers temozolomide resistance by regulating TIMP3/PI3K/AKT axis in gliomas. Molecular Therapy-Oncolytics Mol Ther-Oncolytics. 2021 Sep;22:114 WB Human U87TR cells, U251TR (TMZ-resistant) cells

Zhang, Huijuan, et al. "Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway." Experimental cell research 320.1 (2014): 119-127.

潘晓菲, et al. "长链非编码 RNA CTB-147C22. 8 对复发性呼吸道乳头状瘤上皮细胞内蛋白表达的影响." 中国耳鼻咽喉头颈外科 27.3 (2021): 171.

Flavonoid-Rich Extract of Oldenlandia diffusa (Willd.) Roxb. Inhibits Gastric Cancer by Activation of Caspase-Dependent Mitochondrial Apoptosis Chinese Journal of Integrative Medicine Qiu-lan Wang WB Human

CADM1 impairs the effect of miR-1246 on promoting cell cycle progression in chemo-resistant leukemia cells. BMC CANCER Linjing Li WB Human 1:1000 Blood K562 cell,HL-60 cell

Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation Advanced Science Huan Li IHC Human,Mouse Glioblastoma (GBM) tissue,GBM stem cell-Xenograft

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