CD50 Polyclonal Antibody

    • Catalog No.:YT0767
    • Applications:WB;IF;ELISA
    • Reactivity:Human;Rat;Mouse;
      • Target:
      • CD50/ICAM-3
      • Fields:
      • >>Cell adhesion molecules
      • Gene Name:
      • ICAM3
      • Protein Name:
      • Intercellular adhesion molecule 3
      • Human Gene Id:
      • 3385
      • Human Swiss Prot No:
      • P32942
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human CD50/ICAM-3. AA range:484-533
      • Specificity:
      • CD50 Polyclonal Antibody detects endogenous levels of CD50 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ICAM3;Intercellular adhesion molecule 3;ICAM-3;CDw50;ICAM-R;CD antigen CD50
      • Observed Band(KD):
      • 42-60kD
      • Background:
      • The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016],
      • Function:
      • function:ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.,online information:ICAM-3,PTM:N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.,PTM:Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.,similarity:Belongs to the immunoglobulin superfamily. ICAM family.,similarity:Contains 5 Ig-like C2-type (immunoglobulin-like) domains.,
      • Subcellular Location:
      • Membrane; Single-pass type I membrane protein.
      • Expression:
      • Leukocytes.
      • Products Images
      • Western Blot analysis of NIH-3T3 cells using CD50 Polyclonal Antibody
      • Immunofluorescence analysis of A549 cells, using CD50/ICAM-3 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from NIH/3T3 cells, treated with TNF-a 20ng/ml 5', using CD50/ICAM-3 Antibody. The lane on the right is blocked with the synthesized peptide.