CD32 Polyclonal Antibody
- Catalog No.：YT0754
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Mouse;Rat
- Target：
- CD32
- Fields：
- >>Phagosome;>>Osteoclast differentiation;>>B cell receptor signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Staphylococcus aureus infection;>>Tuberculosis;>>Measles
- Gene Name：
- FCGR2B
- Protein Name：
- Low affinity immunoglobulin gamma Fc region receptor II-b
- Human Gene Id：
- 2213
- Human Swiss Prot No：
- P31994
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human CD32. AA range:277-326
- Specificity：
- CD32 Polyclonal Antibody detects endogenous levels of CD32 protein.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Observed Band(KD)：
- 34kD
- Background：
- The Fc fragment of IgG receptor IIb encoded by FCGR2B is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene.
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- Antibody-FAQs
- Products Images
- Western Blot analysis of SH-SY5Y cells using CD32 Polyclonal Antibody diluted at 1:1000
- Western blot analysis of lysates from 293, HepG2, and HeLa cells, treated with PMA 125ng/ml 30', using CD32 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
