CBG Polyclonal Antibody

    • Catalog No.:YT0689
    • Applications:WB;ELISA
    • Reactivity:Human;Rat;Mouse;
      • Target:
      • CBG
      • Fields:
      • >>Starch and sucrose metabolism;>>Metabolic pathways
      • Gene Name:
      • GBA3
      • Protein Name:
      • Cytosolic beta-glucosidase
      • Human Swiss Prot No:
      • Q9H227
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human GBA3. AA range:291-340
      • Specificity:
      • CBG Polyclonal Antibody detects endogenous levels of CBG protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • GBA3;CBG;CBGL1;Cytosolic beta-glucosidase;Cytosolic beta-glucosidase-like protein 1
      • Observed Band(KD):
      • 54-58kD
      • Background:
      • The protein encoded by this gene is an enzyme that can hydrolyze several types of glycosides. This gene is a polymorphic pseudogene, with the most common allele being the functional allele that encodes the full-length protein. Some individuals, as represented by the reference genome allele, contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2013],
      • Function:
      • catalytic activity:Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.,enzyme regulation:Inhibited by 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside and sodium taurocholate.,function:Glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens.,PTM:The N-terminus is blocked.,similarity:Belongs to the glycosyl hydrolase 1 family.,similarity:Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.,tissue specificity:Present in small intestine (at protein level). Expressed in liver, small intestine, colon, spleen and kidney. Down-regulated in renal cell carcinomas and hepatocellular carcinomas.,
      • Subcellular Location:
      • Cytoplasm, cytosol .
      • Expression:
      • Present in small intestine (at protein level). Expressed in liver, small intestine, colon, spleen and kidney. Down-regulated in renal cell carcinomas and hepatocellular carcinomas.
      • Products Images
      • Western Blot analysis of various cells using CBG Polyclonal Antibody diluted at 1:500
      • Western blot analysis of lysates from HUVEC cells, using GBA3 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from HeLa cells using GBA3 antibody.