CB1 Polyclonal Antibody
- Catalog No.:YT0687
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat;Monkey
- Target:
- CB1
- Fields:
- >>Rap1 signaling pathway;>>Neuroactive ligand-receptor interaction;>>Thermogenesis;>>Retrograde endocannabinoid signaling
- Gene Name:
- CNR1
- Protein Name:
- Cannabinoid receptor 1
- Human Gene Id:
- 1268
- Human Swiss Prot No:
- P21554
- Mouse Gene Id:
- 12801
- Mouse Swiss Prot No:
- P47746
- Rat Gene Id:
- 25248
- Rat Swiss Prot No:
- P20272
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human CNR1. AA range:151-200
- Specificity:
- CB1 Polyclonal Antibody detects endogenous levels of CB1 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- CNR1;CNR;Cannabinoid receptor 1;CB-R;CB1;CANN6
- Observed Band(KD):
- 53kD
- Background:
- This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009],
- Function:
- function:Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.,similarity:Belongs to the G-protein coupled receptor 1 family.,subunit:Interacts (via C-terminus) with CNRIP1.,tissue specificity:Widely expressed.,
- Subcellular Location:
- Cell membrane ; Multi-pass membrane protein . Membrane raft . Mitochondrion outer membrane . Cell projection, axon . Cell junction, synapse, presynapse . Unexpectedly, in the mitochondria, the C-terminus is located in the mitochondrial intermembrane space, a compartment topologically considered as extracellular. In canonical seven-transmembrane G-protein coupled receptors, the C-terminus is cytosolic (By similarity). Found on presynaptic axon terminals in some GABAergic neurons in the somatosensory cortex (By similarity). .
- Expression:
- Widely expressed, with highest levels in fetal and adult brain. Expression levels of isoform 2 and isoform 3 are much lower than those of isoform 1.
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- Antibody-FAQs
- Products Images
- Western Blot analysis of HT-29 cells using CB1 Polyclonal Antibody
- Immunofluorescence analysis of LOVO cells, using CNR1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CNR1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HT-29 and COS7 cells, using CNR1 Antibody. The lane on the right is blocked with the synthesized peptide.