• Target：
• Caspase-3

• Fields：
• >>Platinum drug resistance;>>MAPK signaling pathway;>>p53 signaling pathway;>>Apoptosis;>>Apoptosis - multiple species;>>Natural killer cell mediated cytotoxicity;>>IL-17 signaling pathway;>>TNF signaling pathway;>>Serotonergic synapse;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabetic complications;>>Alcoholic liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Epithelial cell signaling in Helicobacter pylori infection;>>Pathogenic Escherichia coli infection;>>Salmonella infection;>>Pertussis;>>Legionellosis;>>Toxoplasmosis;>>Amoebiasis;>>Tuberculosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Pathways i

• Gene Name：
• CASP3

• Protein Name：
• Caspase3

• Human Gene Id：
• 836

• Human Swiss Prot No：
• P42574

• Mouse Gene Id：
• 12367

• Mouse Swiss Prot No：
• P70677

• Rat Gene Id：
• 25402

• Rat Swiss Prot No：
• P55213

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Caspase 3. AA range:116-165

• Specificity：
• Caspase-3 Polyclonal Antibody detects endogenous levels of Caspase-3 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CASP3;CPP32;Caspase-3;CASP-3;Apopain;Cysteine protease CPP32;CPP-32;Protein Yama;SREBP cleavage activity 1;SCA-1

• Observed Band(KD)：
• 35kD

• Background：
• This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.,enzyme regulation:Inhibited by isatin sulfonamides.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.,PTM:Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Ad

• Subcellular Location：
• Cytoplasm.

• Expression：
• Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Chronic hypoxia and Cu2+ exposure induce gill remodeling of Largemouth bass through endoplasmic reticulum stress, mitochondrial damage and apoptosis AQUATIC TOXICOLOGY Qiao Liu, Hong Wang, Jiayu Ge, Lisen Li, Jie Luo, Kuo He, Haoxiao Yan, Xin Zhang, Rabia Tahir, Wei Luo, Shiyi Chen, Zhang Cheng, Liulan Zhao, Song Yang WB Fish Gill tissue

Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro MICROBIAL PATHOGENESIS Fengyi Zhou, Xin Li, Xiaochi Chang, Zhihao Geng, Wenjing Hao, Jing Deng, Hai Ming Wong, Shuai Wang WB Mouse BMMs

Zhongfeng Capsules protects against cerebral ischemia–reperfusion injury via mediating the phosphoinositide 3-kinase/Akt and toll-like receptor 4/nuclear factor kappa B signaling pathways by regulating neuronal apoptosis and inflammation WB Rat 1:1000 brain tissue/

Synthesis of macromolecular Astragalus polysaccharide-nano selenium complex and the inhibitory effects on HepG2 cells WB Human /HepG2 cell

Melatonin treatment improves human umbilical cord mesenchymal stem cell therapy in a mouse model of type II diabetes mellitus via the PI3K/AKT signaling pathway. Stem Cell Research & Therapy2022 Dec;13(1):1-15. Human,Mouse 1:120 liver tissue hUC-MSC

LncRNA TUG1 Promotes Apoptosis, Invasion, and Angiogenesis of Retinal Endothelial Cells in Retinopathy of Prematurity via MiR-145-5p. Frontiers in Medicine2022 Apr 4;9:803214. Mouse 1:200 retinal tissue

hUC-MSCs lyophilized powder loaded polysaccharide ulvan driven functional hydrogel for chronic diabetic wound healing Carbohyd Polym. 2022 Jul;288:119404. IF Mouse 1:100 wound tissue

The Long-Noncoding RNA TUG1 Regulates Oxygen-Induced Retinal Neovascularization in Mice via MiR-299 Invest Ophth Vis Sci. 2022 Jan;63(1):37-37. WB Mouse 1:1000 retina

The long non-coding RNA PTTG3P promotes cell growth and metastasis via up-regulating PTTG1 and activating PI3K/AKT signaling in hepatocellular carcinoma. Molecular Cancer Mol Cancer. 2018 Dec;17(1):1-16 WB Human HepG2 cell

Chronic chlorpyrifos exposure induces oxidative stress, apoptosis and immune dysfunction in largemouth bass (Micropterus salmoides). ENVIRONMENTAL POLLUTION Environ Pollut. 2021 Aug;282:117010 WB Fish 1:500 Liver

MiR-629-5p Promotes Prostate Cancer Development and Metastasis by Targeting AKAP13. Frontiers in Oncology Front Oncol. 2021; 11: 754353 WB Human LNCaP cell, PC3 cell，C4-2 cell

Antitumor Effect of Fluoxetine on Chronic Stress-Promoted Lung Cancer Growth via Suppressing Kynurenine Pathway and Enhancing Cellular Immunity. Frontiers in Pharmacology Front Pharmacol. 2021 Aug;0:1609 WB Mouse 1:2000 A549 cell-xenograft

Isorhamnetin Promotes MKN-45 Gastric Cancer Cell Apoptosis by Inhibiting PI3K-Mediated Adaptive Autophagy in a Hypoxic Environment. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX WB Human MKN-45 cells

Microcystin-LR Induced Apoptosis in Rat Sertoli Cells via the Mitochondrial Caspase-Dependent Pathway: Role of Reactive Oxygen Species. Frontiers in Physiology 2016 Sep 09 WB Rat Sertoli cell

Primate-specific miR-603 is implicated in the risk and pathogenesis of Alzheimer's disease. Aging-US Aging-Us. 2016 Feb; 8(2): 272–290 WB Human 1:1000 HeLa cell

Suppression of CDCA3 inhibits prostate cancer progression via NF‑κB/cyclin D1 signaling inactivation and p21 accumulation. ONCOLOGY REPORTS Oncol Rep. 2022 Feb;47(2):1-10 WB Human 1:1000 Prostate cancer tissue,paracancerous tissue.

SPAG6 silencing induces apoptosis in the myelodysplastic syndrome cell line SKM‑1 via the PTEN/PI3K/AKT signaling pathway in vitro and in vivo. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2018 Jul;53(1):297-306 IHC Mouse 1:50 SKM-1 cell-Xenograft

SPAG6 silencing induces apoptosis in the myelodysplastic syndrome cell line SKM‑1 via the PTEN/PI3K/AKT signaling pathway in vitro and in vivo. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2018 Jul;53(1):297-306 WB Human 1:500 SKM-1 cell

Emodin Alleviates Intestinal Barrier Dysfunction by Inhibiting Apoptosis and Regulating the Immune Response in Severe Acute Pancreatitis. PANCREAS Pancreas. 2021 Sep;50(8):1202-1211 WB Mouse 1:500 Distal ileum

Emodin Alleviates Intestinal Barrier Dysfunction by Inhibiting Apoptosis and Regulating the Immune Response in Severe Acute Pancreatitis. PANCREAS Pancreas. 2021 Sep;50(8):1202-1211 WB Mouse 1:1000 Distal ileum

Identification of WISP1 as a novel oncogene in glioblastoma. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2017 Oct;51(4):1261-1270 WB Human 1:1000 U251 cell, U373-MG cell

Targeting FoxM1 by thiostrepton inhibits growth and induces apoptosis of laryngeal squamous cell carcinoma. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 2014 Nov 13 WB,IHC Human,Mouse 1:100 LSCC xenograft Hep-2 cell

Aaptamine attenuates the proliferation and progression of non-small cell lung carcinoma. PHARMACEUTICAL BIOLOGY Pharm Biol. 2020;58(1):1044-1054 WB Human A549 cell，H1299 cell

Effects of medicine food Fructus Gardeniae on liver and kidney functions after oral administration to rats for 12 weeks. JOURNAL OF FOOD BIOCHEMISTRY J Food Biochem. 2021 Jul;45(7):e13752 IHC Rat Liver, kidney

Effect of annexin A7 suppression on the apoptosis of gastric cancer cells. MOLECULAR AND CELLULAR BIOCHEMISTRY Mol Cell Biochem. 2017 May;429(1):33-43 WB Human,Mouse BGC823 cell-xenograft BGC823 cell

SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes. ONCOLOGY REPORTS 2017 Mar 31 WB Human 1:500 SKM-1 cell

Ganoderic Acids Prevent Renal Ischemia Reperfusion Injury by Inhibiting Inflammation and Apoptosis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Int J Mol Sci. 2021 Jan;22(19):10229 WB Mouse Renal tissues NRK-52E cell

The inhibitory effects of selenium nanoparticles modified by fructose-enriched polysaccharide from Codonopsis pilosula on HepG2 cells. INDUSTRIAL CROPS AND PRODUCTS Ind Crop Prod. 2022 Feb;176:114335 WB Human HepG2 cell

The role of Se content in improving anti-tumor activities and its potential mechanism for selenized Artemisia sphaerocephala polysaccharides. Food & Function Food Funct. 2021 Mar;12(5):2058-2074 WB Human HepG2 cell

He-Wei granules (HWKL) combat cisplatin-induced nephrotoxicity and myelosuppression in rats by inhibiting oxidative stress, inflammatory cytokines and apoptosis. RSC Advances Rsc Adv. 2017 Mar;7(32):19794-19807 WB Rat kidney

SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis. INTERNATIONAL JOURNAL OF ONCOLOGY 2015 Feb 01 WB Human 1:500 SKM-1 cell， K562 cell

Heme oxygenase-1 (HO-1) protects human lens epithelial cells (SRA01/04) against hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis. EXPERIMENTAL EYE RESEARCH 2016 Mar 16 WB Human 1:2000 LEC cell

Effects of the combination of As2O3 and AZT on proliferation inhibition and apoptosis induction of hepatoma HepG2 cells following silencing of Egr-1. OncoTargets and Therapy Oncotargets Ther. 2018; 11: 3293–3301 WB Human HepG2 cell

Lycium barbarum Polysaccharides Protect Rat Corneal Epithelial Cells against Ultraviolet B-Induced Apoptosis by Attenuating the Mitochondrial Pathway and Inhibiting JNK Phosphorylation. Biomed Research International Biomed Res Int. 2017;2017:5806832 WB Rat 1 : 500 RCE cell

VX-680 induces p53-mediated apoptosis in human cholangiocarcinoma cells. ANTI-CANCER DRUGS Anti-Cancer Drug. 2018 Nov;29(10):1004-1010 WB Human 1 : 1000 HCCC-9810 cell

5,6-Dihydroxy-3,7,4′-trimethoxyflavonol induces G2/M cell cycle arrest and apoptosis in human hepatocellular carcinoma cells. JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH J Asian Nat Prod Res. 2016;18(11):1079-1090 WB Human 1:1000 HepG-2 cell

Recombinant virus expressing hIFN-λ1 (rL-hIFN-λ1) has important effects on endoplasmic reticulum stress, autophagy and apoptosis in small cell lung cancer. Translational Cancer Research Transl Cancer Res. 2020 Sep; 9(9): 5209–5217 WB Human H446 cell

HBx-related long non-coding RNA DBH-AS1 promotes cell proliferation and survival by activating MAPK signaling in hepatocellular carcinoma. Oncotarget Oncotarget. 2015 Oct 20; 6(32): 33791–33804 WB Human HepG2 cell,Hep3B cell

miR-2954 Inhibits PI3K Signaling and Induces Autophagy and Apoptosis in Myocardium Selenium Deficiency. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 2018 Nov 21 WB Chicken 1:500 cardiomyocytes

Overexpression of miR‐200b inhibits the cell proliferation and promotes apoptosis of human hypertrophic scar fibroblasts in vitro. JOURNAL OF DERMATOLOGY WB Human hHSFs cell

Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells Hypoxia/Reoxygenation Injury via Effects on miR-21 Expression, PTEN/Akt, and the Bcl-2/Bax Pathway. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2018;2018:8617314 WB Rat 1:1000 H9c2 cell

The Antitumor Effect of Suicide Gene Therapy Using Bifidobacterium infantis–mediated Herpes Simplex Virus Thymidine Kinase/Ganciclovir in a Nude Mice Model of Renal Cell Carcinoma. UROLOGY 2014 Aug 12 WB Mouse 786-O cell-Xenograft

The preparation of a cold-water soluble polysaccharide from Grifola frondosa and its inhibitory effects on MKN-45 cells. GLYCOCONJUGATE JOURNAL 2020 Jun 15 WB Human MKN-45 cell

Fucoidan inhibits proliferation of the SKM-1 acute myeloid leukaemia cell line via the activation of apoptotic pathways and production of reactive oxygen species. Molecular Medicine Reports Mol Med Rep. 2015 Nov;12(5):6649-6655 WB Human SKM-1 cell

Upregulation of microRNA‑492 induced by epigenetic drug treatment inhibits the malignant phenotype of clear cell renal cell carcinoma in vitro. Molecular Medicine Reports Mol Med Rep. 2015 Jul;12(1):1413-1420 WB Human 1:500 786-O cell, ACHN cell

Mitochondrial separation protein inhibitor inhibits cell apoptosis in rat lungs during intermittent hypoxia. Experimental and Therapeutic Medicine 2019 Mar 01 WB Human,Rat 1:1000 lung WRTL1 cell

Fang, Zhiqing, et al. "Simvastatin inhibits renal cancer cell growth and metastasis via AKT/mTOR, ERK and JAK2/STAT3 pathway." PloS one 8.5 (2013): e62823.

Chen, Yajie, et al. "Down-regulation of BubR1 inhibits growth and induces apoptosis in hepatitis B virus-related hepatocellular carcinoma cells." INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 9.3 (2016): 4129-4139.

5, 6-Dihydroxy-3, 7, 4′-trimethoxyflavonol induces G2/M cell cycle arrest and apoptosis in human hepatocellular carcinoma cells." Journal of Asian natural products research 18.11 (2016): 1079-1090.

向丽萍, et al. "金雀异黄素调控 miR-21 表达促进 LPS 活化的 RAW264. 7 细胞凋亡." 药学学报 54.2 (2019): 281-287.

刘素娟, et al. "染料木素通过 TIPE 2 负性调控 Akt 活性促进脂多糖活化的巨噬细胞凋亡." (2020).

Flavonoid-Rich Extract of Oldenlandia diffusa (Willd.) Roxb. Inhibits Gastric Cancer by Activation of Caspase-Dependent Mitochondrial Apoptosis Chinese Journal of Integrative Medicine Qiu-lan Wang WB Human

The adhesion protein of Mycoplasma genitalium inhibits urethral epithelial cell apoptosis through CypA-CD147 activating PI3K/ Akt/NF-κB pathway APPLIED MICROBIOLOGY AND BIOTECHNOLOGY Yanhua Zeng WB Human

Postsurgical wound management and prevention of triple-negative breast cancer recurrence with a pryoptosis-inducing, photopolymerizable hydrogel JOURNAL OF CONTROLLED RELEASE Sanjun Shi WB Mouse 4 T1 cell

Adhesive Ergothioneine Hyaluronate Gel Protects against Radiation Gastroenteritis by Alleviating Apoptosis, Inflammation, and Gut Microbiota Dysbiosis ACS Applied Materials & Interfaces Yuliang Zhao WB Rat intestinal crypt epithelial (IEC-6) cell cell

Apoptotic vesicles resist oxidative damage in noise-induced hearing loss through activation of FOXO3a-SOD2 pathway Stem Cell Research & Therapy Haidi Yang WB Human Mesenchymal stem cell (MSC),apoptotic vesicles (apoVs)

Comparison of two kinds of Agrocybe cylindracea polysaccharides: structural characteristic and antitumor activity. An-jun Liu WB Human MGC-803 cell

FGF12 regulates cell cycle gene expression and promotes follicular granulosa cell proliferation through ERK phosphorylation in geese. Xingyong Chen WB Geese 1:1000 granulosa cell

The outer membrane protein Tp92 of Treponema pallidum delays human neutrophil apoptosis via the ERK, PI3K/Akt, and NF-κB pathways. MOLECULAR MICROBIOLOGY Yimou Wu WB Human human polymorphonuclear neutrophils (hPMNs)

Protective effect of cornuside on OGD/R injury in SH-SY5Y cells and its underlying mechanism. BRAIN RESEARCH Jianwei Gong WB Human SH-SY5Y cell

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