Calpain 12 Polyclonal Antibody
- Catalog No.:YT0616
- Applications:WB;ELISA
- Reactivity:Human;Mouse
- Target:
- Calpain 12
- Gene Name:
- CAPN12
- Protein Name:
- Calpain-12
- Human Gene Id:
- 147968
- Human Swiss Prot No:
- Q6ZSI9
- Mouse Swiss Prot No:
- Q9ER56
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human CAPN12. AA range:221-270
- Specificity:
- Calpain 12 Polyclonal Antibody detects endogenous levels of Calpain 12 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- CAPN12;Calpain-12;Calcium-activated neutral proteinase 12;CANP 12
- Observed Band(KD):
- 80kD
- Background:
- The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes a member of the calpain large subunit family. [provided by RefSeq, Jun 2012],
- Function:
- catalytic activity:Broad endopeptidase specificity.,function:Calcium-regulated non-lysosomal thiol-protease.,similarity:Belongs to the peptidase C2 family.,similarity:Contains 1 calpain catalytic domain.,similarity:Contains 1 EF-hand domain.,
- Subcellular Location:
- intracellular,cytoplasm,
- Expression:
- Thalamus,
3, 3'-Diindolylmethane induces gastric cancer cells death via STIM1 mediated store-operated calcium entry." International Journal of Biological Sciences 17.5 (2021): 1217.
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using Calpain 12 Polyclonal Antibody
- Western Blot analysis of 293 cells using Calpain 12 Polyclonal Antibody
- Western blot analysis of the lysates from K562 cells using CAPN12 antibody.