• Target：
• CA XII

• Fields：
• >>Nitrogen metabolism;>>Metabolic pathways

• Gene Name：
• CA12

• Protein Name：
• Carbonic anhydrase 12

• Human Gene Id：
• 771

• Human Swiss Prot No：
• O43570

• Mouse Gene Id：
• 76459

• Mouse Swiss Prot No：
• Q8CI85

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human CA12. AA range:231-280

• Specificity：
• CA XII Polyclonal Antibody detects endogenous levels of CA XII protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC: 100-300.WB 1:500 - 1:2000. ELISA: 1:40000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CA12;Carbonic anhydrase 12;Carbonate dehydratase XII;Carbonic anhydrase XII;CA-XII;Tumor antigen HOM-RCC-3.1.3

• Observed Band(KD)：
• 40kD

• Background：
• Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014],

• Function：
• catalytic activity:H(2)CO(3) = CO(2) + H(2)O.,cofactor:Zinc.,enzyme regulation:Inhibited by acetazolamide.,function:Reversible hydration of carbon dioxide.,similarity:Belongs to the alpha-carbonic anhydrase family.,tissue specificity:Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis.,

• Subcellular Location：
• Membrane; Single-pass type I membrane protein. Cell membrane .

• Expression：
• Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium (PubMed:26911677).

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