BRD3 Polyclonal Antibody

    • Catalog No.:YT0522
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • BRD3
      • Gene Name:
      • BRD3
      • Protein Name:
      • Bromodomain-containing protein 3
      • Human Gene Id:
      • 8019
      • Human Swiss Prot No:
      • Q15059
      • Mouse Swiss Prot No:
      • Q8K2F0
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human BRD3. AA range:611-660
      • Specificity:
      • BRD3 Polyclonal Antibody detects endogenous levels of BRD3 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • BRD3;KIAA0043;RING3L;Bromodomain-containing protein 3;RING3-like protein
      • Observed Band(KD):
      • 90kD
      • Background:
      • This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq, Jul 2008],
      • Function:
      • disease:A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.,similarity:Contains 2 bromo domains.,tissue specificity:Ubiquitous.,
      • Subcellular Location:
      • Nucleus . Detected on chromatin. .
      • Expression:
      • Ubiquitous.
      • Products Images
      • Western Blot analysis of various cells using BRD3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Western Blot analysis of A549 cells using BRD3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).