BRD3 Polyclonal Antibody
- Catalog No.:YT0522
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- BRD3
- Gene Name:
- BRD3
- Protein Name:
- Bromodomain-containing protein 3
- Human Gene Id:
- 8019
- Human Swiss Prot No:
- Q15059
- Mouse Gene Id:
- 67382
- Mouse Swiss Prot No:
- Q8K2F0
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human BRD3. AA range:611-660
- Specificity:
- BRD3 Polyclonal Antibody detects endogenous levels of BRD3 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- BRD3;KIAA0043;RING3L;Bromodomain-containing protein 3;RING3-like protein
- Observed Band(KD):
- 90kD
- Background:
- This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq, Jul 2008],
- Function:
- disease:A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.,similarity:Contains 2 bromo domains.,tissue specificity:Ubiquitous.,
- Subcellular Location:
- Nucleus . Detected on chromatin. .
- Expression:
- Ubiquitous.
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using BRD3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western Blot analysis of A549 cells using BRD3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).