• Target：
• BCKDK

• Gene Name：
• BCKDK

• Protein Name：
• [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase mitochondrial

• Human Gene Id：
• 10295

• Human Swiss Prot No：
• O14874

• Mouse Gene Id：
• 12041

• Mouse Swiss Prot No：
• O55028

• Rat Gene Id：
• 29603

• Rat Swiss Prot No：
• Q00972

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human BCKD . AA range:11-60

• Specificity：
• BCKDK Polyclonal Antibody detects endogenous levels of BCKDK protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• BCKDK;[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase; mitochondrial;Branched-chain alpha-ketoacid dehydrogenase kinase;BCKD-kinase;BCKDHKIN

• Observed Band(KD)：
• 46kD

• Background：
• The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012],

• Function：
• catalytic activity:ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] = ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate.,function:Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.,PTM:Autophosphorylated.,similarity:Belongs to the PDK/BCKDK protein kinase family.,similarity:Contains 1 histidine kinase domain.,subunit:Monomer.,tissue specificity:Ubiquitous.,

• Subcellular Location：
• Mitochondrion matrix. Mitochondrion .

• Expression：
• Ubiquitous.

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