• Target：
• ATG4b

• Fields：
• >>Autophagy - other;>>Autophagy - animal

• Gene Name：
• ATG4B

• Protein Name：
• Cysteine protease ATG4B

• Human Gene Id：
• 23192

• Human Swiss Prot No：
• Q9Y4P1

• Mouse Gene Id：
• 66615

• Mouse Swiss Prot No：
• Q8BGE6

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human ATG4B. AA range:71-120

• Specificity：
• Atg4b Polyclonal Antibody detects endogenous levels of Atg4b protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ATG4B;APG4B;AUTL1;KIAA0943;Cysteine protease ATG4B;AUT-like 1 cysteine endopeptidase;Autophagin-1;Autophagy-related cysteine endopeptidase 1;Autophagy-related protein 4 homolog B;hAPG4B

• Observed Band(KD)：
• 44kD

• Background：
• Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],

• Function：
• enzyme regulation:Inhibited by N-ethylmaleimide.,function:Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.,similarity:Belongs to the peptidase C54 family.,tissue specificity:Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.,

• Subcellular Location：
• Cytoplasm . Cytoplasm, cytosol . Cytoplasmic vesicle, autophagosome . Endoplasmic reticulum . Mitochondrion . Mainly localizes to the cytoplasm, including cytosol (PubMed:29165041). A samll potion localizes to mitochondria; phosphorylation at Ser-34 promotes localization to mitochondria (PubMed:29165041). .

• Expression：
• Brain,Embryo,Endometrium,Epithelium,Hippocampus,Liver,Placenta,Test

Methylation-induced silencing of miR-34a enhances chemoresistance by directly upregulating ATG4B-induced autophagy through AMPK/mTOR pathway in prostate cancer. ONCOLOGY REPORTS 2015 Oct 16 WB Human PC-3 cell, DU145 cell

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