AR-α1B Polyclonal Antibody
- Catalog No.:YT0356
- Applications:WB;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- AR-α1B
- Fields:
- >>Calcium signaling pathway;>>cGMP-PKG signaling pathway;>>Neuroactive ligand-receptor interaction;>>Adrenergic signaling in cardiomyocytes;>>Vascular smooth muscle contraction;>>Salivary secretion
- Gene Name:
- ADRA1B
- Protein Name:
- Alpha-1B adrenergic receptor
- Human Gene Id:
- 147
- Human Swiss Prot No:
- P35368
- Mouse Swiss Prot No:
- P97717
- Rat Swiss Prot No:
- P15823
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human ADRA1B. AA range:431-480
- Specificity:
- AR-α1B Polyclonal Antibody detects endogenous levels of AR-α1B protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- ADRA1B;Alpha-1B adrenergic receptor;Alpha-1B adrenoreceptor;Alpha-1B adrenoceptor
- Observed Band(KD):
- 60kD
- Background:
- Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This gene comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region. [provided by RefSeq, Jul 2008],
- Function:
- function:This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.,similarity:Belongs to the G-protein coupled receptor 1 family.,
- Subcellular Location:
- Nucleus membrane; Multi-pass membrane protein. Cell membrane ; Multi-pass membrane protein . Cytoplasm . Membrane, caveola . Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
- Expression:
- Brain,
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- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of JK cells using AR-α1B Polyclonal Antibody
- Immunofluorescence analysis of LOVO cells, using ADRA1B Antibody. The picture on the right is blocked with the synthesized peptide.