Akt Polyclonal Antibody

    • Catalog No.:YT0185
    • Applications:IF;WB;IHC;ELISA
    • Reactivity:Human;Mouse;Rat;Chicken
      • Target:
      • AKT1/2/3
      • Fields:
      • >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>Platinum drug resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Adrenergic signaling in cardiomyocytes;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Signaling pathways regulating pluripotency of stem cells;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>JAK-STAT signaling pathway;>>T cell recept
      • Gene Name:
      • AKT1/AKT2/AKT3
      • Protein Name:
      • RAC-alpha serine/threonine-protein kinase/RAC-beta serine/threonine-protein kinase/RAC-gamma serine/threonine-protein kinase
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human AKT1/2/3. AA range:281-330
      • Specificity:
      • Akt Polyclonal Antibody detects endogenous levels of Akt protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • AKT1;PKB;RAC;RAC-alpha serine/threonine-protein kinase;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;Proto-oncogene c-Akt;RAC-PK-alpha;AKT2;RAC-beta serine/threonine-protein kinase;Protein kinase Akt-2;Protein kinase B
      • Observed Band(KD):
      • 56kD
      • Background:
      • The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in AKT1 are associated with breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime.,disease:Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].,disease:Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).,domain:Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.,domain:The AGC-kinase C-terminal mediates interaction with THEM4.,enzyme regulation:Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.,function:Gene
      • Subcellular Location:
      • Cytoplasm . Nucleus . Cell membrane . Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (PubMed:16792529). .
      • Expression:
      • Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.

      CFNC, a neocryptolepine derivative, inhibited the growth of gastric cancer AGS cells by inhibiting PI3K/AKT signaling pathway

      Combination of chloroquine diphosphate and salidroside induces human liver cell apoptosis via regulation of mitochondrial dysfunction and autophagy Molecular Medicine Reports Bing Jiang, Longfei Feng, Tao Yang, Wenjing Guo, Yangyang Li, Tao Wang, Chengguang Liu, Haixiang Su WB Human 97H cell

      MicroRNA-10 Family Promotes the Epithelial-to-Mesenchymal Transition in Renal Fibrosis by the PTEN/Akt Pathway CURRENT ISSUES IN MOLECULAR BIOLOGY Chaokun Wang, Yichen Shuai, Chuan Zhao, Fengrui Yang, Weilian Su, Zhifen Ning, Guoxia Li WB Human HK-2 cell

      Ursolic acid inhibits the activation of smoothened-independent non-canonical hedgehog pathway in colorectal cancer by suppressing AKT signaling cascade WB Human /HCT-116 cells

      Zhongfeng Capsules protects against cerebral ischemia–reperfusion injury via mediating the phosphoinositide 3-kinase/Akt and toll-like receptor 4/nuclear factor kappa B signaling pathways by regulating neuronal apoptosis and inflammation WB Rat 1:1000 brain tissue/

      Synthesis and anticancer evaluations of novel 1H-imidazole [4,5-f][1,10] phenanthroline derivative for the treatment of colorectal cancer WB Human /HCT116, SW480 cells

      Proteomic Study on the Reproductive Toxicity of Tripterygium Glycosides in Rats WB Rat testis tissue /

      Melatonin treatment improves human umbilical cord mesenchymal stem cell therapy in a mouse model of type II diabetes mellitus via the PI3K/AKT signaling pathway. Stem Cell Research & Therapy2022 Dec;13(1):1-15. Human,Mouse 1:1200 liver tissue hUC-MSC

      Design, synthesis, and cytotoxic activities of isaindigotone derivatives as potential anti-gastric cancer agents. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY2022;37(1):1212-1226. Human AGS cell

      Licoflavone A Suppresses Gastric Cancer Growth and Metastasis by Blocking the VEGFR-2 Signaling Pathway. Journal of Oncology2022;2022:5497991. Human 1 : 1000 MKN-45 cell

      An Integrated Approach Based on Network Analysis Combined With Experimental Verification Reveals PI3K/Akt/Nrf2 Signaling Is an Important Way for the Anti-Myocardial Ischemia Activity of Yi-Qi-Tong-Luo Capsule. Front Pharmacol. 2022 Feb;13:794528-794528. WB,IF Rat 1:1000

      Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis. NATURE STRUCTURAL & MOLECULAR BIOLOGY 2016 Apr 11 WB Human gastric cancer tissues

      Deletion of FGF9 in GABAergic neurons causes epilepsy. Cell Death & Disease Cell Death Dis. 2021 Feb;12(2):1-13 WB Mouse Brain

      3-Epipachysamine B suppresses proliferation and induces apoptosis of breast cancer cell via PI3K/AKT/mTOR signaling pathway. LIFE SCIENCES Life Sci. 2021 Nov;285:119995 WB Human 1:800 MCF-7 cell,MDA-MB-231 cell

      Dexamethasone Protects Against Ischaemic Brain Injury via Inhibiting the pAkt Signalling Pathway Through Increasing Hap1. NEUROTOXICITY RESEARCH 2020 Sep 02 WB Mouse 1 : 500 Brain

      Autophagy and apoptosis mediated nano-copper-induced testicular damage. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2022 Jan;229:113039 WB Rat Testis

      circCDYL2, Overexpressed in Highly Migratory Colorectal Cancer Cells, Promotes Migration by Binding to Ezrin. Frontiers in Oncology Front Oncol. 2021; 11: 716073 WB,IHC Human 1:1000 SW620, RKOSW480,HCT116

      Loss of Tctn3 causes neuronal apoptosis and neural tube defects in mice. Cell Death & Disease Cell Death Dis. 2018 May;9(5):1-12 WB Mouse 1:1000 brain

      Tyrosine nitrations impaired intracellular trafficking of FSHR to the cell surface and FSH-induced Akt-FoxO3a signaling in human granulosa cells. Aging-US Aging-Us. 2019 May 31; 11(10): 3094–3116 WB Human granulosa cell (GC)

      Mn-TAT PTD-Ngb attenuates oxidative injury by an enhanced ROS scavenging ability and the regulation of redox signaling pathway. Scientific Reports Sci Rep-Uk. 2019 Dec;9(1):1-12 WB Rat 1:1000 PC12 cell

      Cystamine attenuated behavioral deficiency via increasing the expression of BDNF and activating PI3K/Akt signaling in 2,5-hexanedione intoxicated rats. Toxicology Research 2017 Mar 01 WB Rat spinal cord

      Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH J Exp Clin Canc Res. 2017 Dec;36(1):1-13 WB Human 1:2000 HepG2 cell, Huh-7 cell

      Pirfenidone decreases mesothelioma cell proliferation and migration via inhibition of ERK and AKT and regulates mesothelioma tumor microenvironment in vivo. Scientific Reports 2018 Jul 03 WB Human JL-1 cell,H2052 cell

      CD38 is involved in cell energy metabolism via activating the PI3K/AKT/mTOR signaling pathway in cervical cancer cells. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2020 Jul;57(1):338-354 WB Human 1:1000 CaSki cell,Hela cell

      Negative Regulation of Grb10 Interacting GYF Protein 2 on Insulin-Like Growth Factor-1 Receptor Signaling Pathway Caused Diabetic Mice Cognitive Impairment. PLoS One 2014 Sep 30 WB Mouse 1∶1000 hippocampus

      Inactivation of the Phosphatidylinositol 3‐Kinase/Akt Pathway is Involved in BMP9‐mediated Tumor‐suppressive Effects in Gastric Cancer Cells. JOURNAL OF CELLULAR BIOCHEMISTRY 2015 Apr 10 WB Human 1:1000 gastric cancer tissue SGC-7901 cell, MNK-45 cell

      FGF9 knockout in GABAergic neurons induces apoptosis and inflammation via the Fas/caspase-3 pathway in the cerebellum of mice. BRAIN RESEARCH BULLETIN 2019 Nov 11 WB Mouse tail tissue

      Higenamine enhances the antitumor effects of cucurbitacin B in breast cancer by inhibiting the interaction of AKT and CDK2. ONCOLOGY REPORTS Oncol Rep. 2018 Oct;40(4):2127-2136 WB Human 1:1000 T47D cell, SKBr3 cell

      CD90 affects the biological behavior and energy metabolism level of gastric cancer cells by targeting the PI3K/AKT/HIF‑1α signaling pathway. Oncology Letters Oncol Lett. 2021 Mar;21(3):1-1 WB Human 1:1000 AGS cell

      FGF9 modulates Schwann cell myelination in developing nerves and induces a pro‐inflammatory environment during injury. JOURNAL OF CELLULAR BIOCHEMISTRY J Cell Biochem. 2018 Oct;119(10):8643-8658 WB Mouse sciatic nerves

      Exogenous Adipokine Peptide Resistin Protects Against Focal Cerebral Ischemia/Reperfusion Injury in Mice. NEUROCHEMICAL RESEARCH Neurochem Res. 2017 Oct;42(10):2949-2957 WB Mouse right hemisphere

      scAAV9-VEGF prolongs the survival of transgenic ALS mice by promoting activation of M2 microglia and the PI3K/Akt pathway. BRAIN RESEARCH Brain Res. 2016 Oct;1648:1 WB Mouse 1:500 spinal

      Intrathecal Delivery of ssAAV9-DAO Extends Survival in SOD1G93A ALS Mice. NEUROCHEMICAL RESEARCH Neurochem Res. 2017 Apr;42(4):986-996 WB Mouse spinal cord

      Protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H2O2‑induced oxidative damage and apoptosis in vitro. Experimental and Therapeutic Medicine Exp Ther Med. 2021 Dec;22(6):1-11 WB Rat 1:1000 Rat aortic endothelial cells (RAECs)

      Ad-p53 enhances the sensitivity of triple-negative breast cancer MDA-MB-468 cells to the EGFR inhibitor gefitinib. ONCOLOGY REPORTS 2014 Dec 11 WB Human MDA-MB-468 cell

      Overexpression of ATG4a promotes autophagy and proliferation, and inhibits apoptosis in lens epithelial cells via the AMPK and Akt pathways. Molecular Medicine Reports Mol Med Rep. 2020 Aug;22(2):1295-1302 WB Human 1 : 1000 Lens epithelial cells

      Polygonum cuspidatum Extract Exerts Antihyperlipidemic Effects by Regulation of PI3K/AKT/FOXO3 Signaling Pathway. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2021;2021:3830671 IF,WB Human 1:1000 HepG2 cells

      Autophagy was activated against the damages of placentas caused by nano-copper oral exposure. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2021 Sep;220:112364 WB Rat 1 : 1000 Placentas

      Induction of PI3K/Akt-Mediated Apoptosis in Osteoclasts Is a Key Approach for Buxue Tongluo Pills to Treat Osteonecrosis of the Femoral Head.. Frontiers in Pharmacology Front Pharmacol. 2021 Nov;12:729909-729909 WB Mouse 1:1000 RAW 264.7 cell

      Intramuscular Delivery of scAAV9-hIGF1 Prolongs Survival in the hSOD1 G93A ALS Mouse Model via Upregulation of D-Amino Acid Oxidase. MOLECULAR NEUROBIOLOGY Mol Neurobiol. 2018 Jan;55(1):682-695 WB Mouse 1:1000 lumbar spinal cord tissue

      PRMT5 promotes epithelial‐mesenchymal transition via EGFR‐β‐catenin axis in pancreatic cancer cells. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 2019 Dec 18 WB Human PaTu8988 cell, SW1990 cell

      Cadmium induces BNIP3-dependent autophagy in chicken spleen by modulating miR-33-AMPK axis. CHEMOSPHERE 2017 Dec 06 WB Chicken 1:500 Spleen

      Adropin reduces hypoxia/reoxygenation‑induced myocardial injury via the reperfusion injury salvage kinase pathway. Experimental and Therapeutic Medicine Exp Ther Med. 2019 Nov;18(5):3307-3314 WB Rat 1:1000 H9c2 cell

      HSP90 inhibitor AUY922 can reverse Fulvestrant induced feedback reaction in human breast cancer cells. CANCER SCIENCE 2017 May 19 WB Human MCF-7 cell,T47D cell

      PDGF-mediated PI3K/AKT/β-catenin signaling regulates gap junctions in corpus cavernosum smooth muscle cells. EXPERIMENTAL CELL RESEARCH Exp Cell Res. 2018 Jan;362:252 WB Rat 1:1000 corpus cavernosum smooth muscle cells (CCSMCs)

      5‐FU inhibits migration and invasion of CRC cells through PI3K/AKT pathway regulated by MARCH1. CELL BIOLOGY INTERNATIONAL Cell Biol Int. 2021 Feb;45(2):368-381 WB Human Colorectal cancer(CRC ) tissues sw480s,DLD-1s,NCM460s

      The PI3K/p-Akt signaling pathway participates in calcitriol ameliorating podocyte injury in DN rats. METABOLISM-CLINICAL AND EXPERIMENTAL Metabolism. 2014 Oct;63:1324 WB Rat Renal tissues

      HMGB1 binding to receptor for advanced glycation end products enhances inflammatory responses of human bronchial epithelial cells by activating p38 MAPK and ERK1/2. MOLECULAR AND CELLULAR BIOCHEMISTRY Mol Cell Biochem. 2015 Jul;405(1):63-71 WB Human HBECs

      Effects of enriched environment on depression and anxiety-like behavior induced by early life stress: A comparison between different periods. BEHAVIOURAL BRAIN RESEARCH Behav Brain Res. 2021 Aug;411:113389 WB Mouse 1 : 1000 Hippocampal tissue

      MicroRNA-32 promotes calcification in vascular smooth muscle cells: Implications as a novel marker for coronary artery calcification. PLoS One Plos One. 2017 Mar;12(3):e0174138 WB Mouse MVSMCs

      An Integrated Approach Based on Network Pharmacology Combined with Experimental Verification Reveals AMPK/PI3K/Akt Signaling is an Important Way for the Anti-Type 2 Diabetic Activity of Silkworm Excrement. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy Diabet Metab Synd Ob. 2021 Feb;14:601-616 WB Human 1:1000 HepG2 cell

      CCL21/CCR7 interaction promotes cellular migration and invasion via modulation of the MEK/ERK1/2 signaling pathway and correlates with lymphatic metastatic spread and poor prognosis in urinary bladder cancer. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2017 Jul;51(1):75-90 WB Human 1:2000 UM-UC-3 cell

      Silver Nanoparticles Induced Oxidative Stress and Mitochondrial Injuries Mediated Autophagy in HC11 Cells Through Akt/AMPK/mTOR Pathway. BIOLOGICAL TRACE ELEMENT RESEARCH Biol Trace Elem Res. 2021 Mar;199(3):1062-1073 WB Human 1:1000 HC11 cell

      Gastrointestinal Factor GDDR Attenuates Epithelial–Mesenchymal Transition in Gastric Cancer via Inhibiting AKT Signal. DIGESTIVE DISEASES AND SCIENCES Digest Dis Sci. 2016 Jul;61(7):1941-1949 WB Human gastric cancer tissues SGC7901 cell

      Cardamonin inhibits angiotensin II-induced vascular smooth muscle cell proliferation and migration by downregulating p38 MAPK, Akt, and ERK phosphorylation. Journal of Natural Medicines J Nat Med-Tokyo. 2014 Jul;68(3):623-629 WB Rat VSMCs

      Hydroxysafflor Yellow A Attenuates Hydrogen Peroxide-Induced Oxidative Damage on Human Umbilical Vein Endothelial Cells. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2020;2020:8214128 WB Human 1:2000 HUVECs

      MicroRNA-7-5p regulates human alveolar epithelial sodium channels by targeting the mTORC2/SGK-1 signaling pathway. EXPERIMENTAL LUNG RESEARCH 2016 Jun 22 WB Human 1:1000 A549 cell

      Tian, Sha-Sha, et al. "Flavonoids from the leaves of Carya cathayensis Sarg. inhibit vascular endothelial growth factor-induced angiogenesis." Fitoterapia 92 (2014): 34-40.

      Wang, Wei-wei, et al. "Telmisartan reduces atrial arrhythmia susceptibility through the regulation of RAS–ERK and PI3K–Akt–eNOS pathways in spontaneously hypertensive rats." Canadian journal of physiology and pharmacology 93.8 (2015): 657-665.

      Cao, Zheng, et al. "Exendin-4 attenuates high glucose-induced endothelial progenitor cells dysfunction through the PI3-K/Akt/eNOS pathway." INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 10.6 (2017): 6774-6780.

      佟雪, et al. "基于 PI3K/AKT/mTOR 信号通路研究补肾法对体外培养小鼠卵母细胞成熟的促进作用." 北京中医药大学学报 44.6 (2021): 510-518.

      Zhang, Xianxie, et al. "Salvia miltiorrhiza alleviates hypoxia-induced nerve injury associated with Ngb/Akt intracellular signaling pathway." (2020).

      刘素娟, et al. "染料木素通过 TIPE 2 负性调控 Akt 活性促进脂多糖活化的巨噬细胞凋亡." (2020).

      Liu, Qi, et al. "Role of miR-731 and miR-2188-3p in mediating Chlorpyrifos induced head kidney injury in common carp via targeting TLR and apoptosis pathways." Aquatic Toxicology (2019): 105286.

      恪雪, et al. "基于 PI3K/AKT/mTOR 信号通路研究补肾法对体外培养 小鼠卵母细胞成熟的促进作." Journal of Beijing University of Traditional Chinese Medicine 44.6 (2021).

      Cadmium induces BNIP3-dependent autophagy in chicken spleen by modulating miR-33-AMPK axis. CHEMOSPHERE 2017 Dec 06 WB Chicken 1:1000 Spleen

      5‐FU inhibits migration and invasion of CRC cells through PI3K/AKT pathway regulated by MARCH1. CELL BIOLOGY INTERNATIONAL Cell Biol Int. 2021 Feb;45(2):368-381 WB Human Colorectal cancer(CRC ) tissues sw480s,DLD-1s,NCM460s

      Dexamethasone Protects Against Ischaemic Brain Injury via Inhibiting the pAkt Signalling Pathway Through Increasing Hap1. NEUROTOXICITY RESEARCH 2020 Sep 02 WB Mouse 1 : 500 Brain

      COX-2/sEH Dual Inhibitor Alleviates Hepatocyte Senescence in NAFLD Mice by Restoring Autophagy through Sirt1/PI3K/AKT/mTOR INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Chen-Yu Zhang WB Mouse

      The Hypoglycemic Effect of JinQi Jiangtang Tablets Is Partially Dependent on the Palmatine-Induced Activation of the Fibroblast Growth Factor Receptor 1 Signaling Pathway Frontiers in Pharmacology Yubin Ji WB Mouse,Human

      Arsenic Trioxide-loaded nanoparticles Enhance the Chemosensitivity of Gemcitabine in Pancreatic Cancer via Reversal of Pancreatic Stellate Cells Desmoplasia through Targeting AP4/Galectin-1 Pathway Biomaterials Science Kaihong Huang WB Human

      6-Methoxydihydrosanguinarine induces apoptosis and autophagy in breast cancer MCF-7 cells by accumulating ROS to suppress the PI3K/AKT/mTOR signaling pathway PHYTOTHERAPY RESEARCH Hui Zhang WB Human

      The adhesion protein of Mycoplasma genitalium inhibits urethral epithelial cell apoptosis through CypA-CD147 activating PI3K/ Akt/NF-κB pathway APPLIED MICROBIOLOGY AND BIOTECHNOLOGY Yanhua Zeng WB Human

      Nanocarrier of Pin1 inhibitor based on supercritical fluid technology inhibits cancer metastasis by blocking multiple signaling pathways Regenerative Biomaterials Dayun Yang WB Human,Mouse lung tissues HuH7 cell,HepG2 cell

      UQCRFS1 serves as a prognostic biomarker and promotes the progression of ovarian cancer. Jing Wang WB Human 1:1000 ovarian tissue A2780 cell,OVCAR8 cell

      Hepatoprotective efficacy and interventional mechanism of JianPi LiShi YangGan formula in acute-on-chronic liver failure. Xiaozhou Zhou IHC,WB Mouse 1:200,1:1000 liver AML12 cell

      Quercetin inhibits mesothelial-mesenchymal transition and alleviates postoperative peritoneal adhesions by blocking the TGF-β1/PI3K/AKT pathway. JOURNAL OF ETHNOPHARMACOLOGY Zhengdong Jiang WB Human 1:2000 HMrSV5 cell

      Crosstalk between autophagy inhibitor and salidroside-induced apoptosis: A novel strategy for autophagy-based treatment of hepatocellular cancer. INTERNATIONAL IMMUNOPHARMACOLOGY Haixiang Su WB Human 1:1000 HepG2 cell,97H cell

      Fecal microbiota transplantation and short-chain fatty acids improve learning and memory in fluorosis mice by BDNF-PI3K/AKT pathway. CHEMICO-BIOLOGICAL INTERACTIONS Ruiyan Niu WB Mouse 1: 2000 hippocampus

      • Products Images
      • Xiong, Yang, et al. "CCL21/CCR7 interaction promotes cellular migration and invasion via modulation of the MEK/ERK1/2 signaling pathway and correlates with lymphatic metastatic spread and poor prognosis in urinary bladder cancer." International journal of oncology 51.1 (2017): 75-90.
      • Wang, Xinzhao, et al. "Ad-p53 enhances the sensitivity of triple-negative breast cancer MDA-MB-468 cells to the EGFR inhibitor gefitinib." Oncology reports 33.2 (2015): 526-532.
      • Liu, Jianghua, et al. "MicroRNA-32 promotes calcification in vascular smooth muscle cells: Implications as a novel marker for coronary artery calcification." PloS one 12.3 (2017): e0174138.
      • Immunofluorescence analysis of human-stomach tissue. 1,Akt Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of rat-lung tissue. 1,Akt Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of mouse-spleen tissue. 1,Akt Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
      • Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
      • Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,Akt Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1,Akt Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AKT1/2/3 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from 1) 293T, 2) L929, 3) COLO, 4) Hela cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (cat:YM3028) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour.