Actin Polyclonal Antibody

    • Catalog No.:YT0096
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat;Fish
      • Target:
      • Actin pan
      • Fields:
      • >>Rap1 signaling pathway;>>Phagosome;>>Apoptosis;>>Hippo signaling pathway;>>Focal adhesion;>>Adherens junction;>>Tight junction;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Leukocyte transendothelial migration;>>Thermogenesis;>>Regulation of actin cytoskeleton;>>Thyroid hormone signaling pathway;>>Oxytocin signaling pathway;>>Gastric acid secretion;>>Amyotrophic lateral sclerosis;>>Bacterial invasion of epithelial cells;>>Vibrio cholerae infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection;>>Influenza A;>>Proteoglycans in cancer;>>Hepatocellular carcinoma;>>Hypertrophic cardiomyopathy;>>Arrhythmogenic right ventricular cardiomyopathy;>>Dilated cardiomyopathy;>>Viral myocarditis;>>Fluid shear stress and atherosclerosis
      • Gene Name:
      • ACTG1
      • Protein Name:
      • Actin cytoplasmic 2
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Actin. AA range:326-375
      • Specificity:
      • Actin Polyclonal Antibody detects endogenous levels of Actin protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ACTB;Actin; cytoplasmic 1;Beta-actin;POTEKP;ACTBL3;FKSG30;Putative beta-actin-like protein 3;Kappa-actin;POTE ankyrin domain family member K;ACTG1;ACTB;ACTG;Actin, cytoplasmic 2;Gamma-actin
      • Observed Band(KD):
      • 45kD
      • Background:
      • This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
      • Function:
      • disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the
      • Subcellular Location:
      • Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
      • Expression:
      • B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car
      • Products Images
      • Western Blot analysis of various cells using Actin Polyclonal Antibody diluted at 1:2000
      • Western Blot analysis of HeLa cells using Actin Polyclonal Antibody diluted at 1:2000
      • Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Actin-pan Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HeLa cells, using Actin-pan Antibody. The lane on the right is blocked with the synthesized peptide.