ACOT4 Polyclonal Antibody
- Catalog No.:YT0088
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- ACOT4
- Fields:
- >>Fatty acid elongation;>>Biosynthesis of unsaturated fatty acids;>>Metabolic pathways;>>Ovarian steroidogenesis
- Gene Name:
- ACOT4
- Protein Name:
- Acyl-coenzyme A thioesterase 4
- Human Gene Id:
- 122970
- Human Swiss Prot No:
- Q8N9L9
- Mouse Swiss Prot No:
- Q8BWN8
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human ACOT4. AA range:361-410
- Specificity:
- ACOT4 Polyclonal Antibody detects endogenous levels of ACOT4 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- ACOT4;PTE2B;PTEIB;Acyl-coenzyme A thioesterase 4;Acyl-CoA thioesterase 4;PTE-2b;Peroxisomal acyl coenzyme A thioester hydrolase Ib;Peroxisomal long-chain acyl-CoA thioesterase Ib;PTE-Ib
- Observed Band(KD):
- 48kD
- Background:
- catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.,similarity:Belongs to the C/M/P thioester hydrolase family.,
- Function:
- catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.,similarity:Belongs to the C/M/P thioester hydrolase family.,
- Subcellular Location:
- Peroxisome .
- Expression:
- Strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle.
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using ACOT4 Polyclonal Antibody diluted at 1:1000
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Immunofluorescence analysis of MCF7 cells, using ACOT4 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HeLa and COLO cells, using ACOT4 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from COLO205 cells using ACOT4 antibody.