ACOT4 Polyclonal Antibody

    • Catalog No.:YT0088
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • ACOT4
      • Fields:
      • >>Fatty acid elongation;>>Biosynthesis of unsaturated fatty acids;>>Metabolic pathways;>>Ovarian steroidogenesis
      • Gene Name:
      • ACOT4
      • Protein Name:
      • Acyl-coenzyme A thioesterase 4
      • Human Swiss Prot No:
      • Q8N9L9
      • Mouse Swiss Prot No:
      • Q8BWN8
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ACOT4. AA range:361-410
      • Specificity:
      • ACOT4 Polyclonal Antibody detects endogenous levels of ACOT4 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ACOT4;PTE2B;PTEIB;Acyl-coenzyme A thioesterase 4;Acyl-CoA thioesterase 4;PTE-2b;Peroxisomal acyl coenzyme A thioester hydrolase Ib;Peroxisomal long-chain acyl-CoA thioesterase Ib;PTE-Ib
      • Observed Band(KD):
      • 48kD
      • Background:
      • catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.,similarity:Belongs to the C/M/P thioester hydrolase family.,
      • Function:
      • catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.,similarity:Belongs to the C/M/P thioester hydrolase family.,
      • Subcellular Location:
      • Peroxisome .
      • Expression:
      • Strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle.
      • Products Images
      • Western Blot analysis of various cells using ACOT4 Polyclonal Antibody diluted at 1:1000
      • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Immunofluorescence analysis of MCF7 cells, using ACOT4 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HeLa and COLO cells, using ACOT4 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from COLO205 cells using ACOT4 antibody.