ACOT12 Polyclonal Antibody
- Catalog No.:YT0086
- Applications:IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- ACOT12
- Fields:
- >>Pyruvate metabolism;>>Metabolic pathways
- Gene Name:
- ACOT12
- Protein Name:
- Acyl-coenzyme A thioesterase 12
- Human Gene Id:
- 134526
- Human Swiss Prot No:
- Q8WYK0
- Mouse Gene Id:
- 74156
- Mouse Swiss Prot No:
- Q9DBK0
- Rat Gene Id:
- 170570
- Rat Swiss Prot No:
- Q99NB7
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human ACOT12. AA range:281-330
- Specificity:
- ACOT12 Polyclonal Antibody detects endogenous levels of ACOT12 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- ACOT12;CACH;CACH1;STARD15;Acyl-coenzyme A thioesterase 12;Acyl-CoA thioesterase 12;Acyl-CoA thioester hydrolase 12;Cytoplasmic acetyl-CoA hydrolase 1;CACH-1;hCACH-1;START domain-containing protein 15;StARD15
- Molecular Weight(Da):
- 62kD
- Background:
- catalytic activity:Acetyl-CoA + H(2)O = CoA + acetate.,function:Hydrolyzes acetyl-CoA to acetate and CoA.,pathway:Carbohydrate metabolism; pyruvate metabolism.,similarity:Contains 1 START domain.,similarity:Contains 2 acyl coenzyme A hydrolase domains.,subunit:Homodimer or homotetramer.,
- Function:
- catalytic activity:Acetyl-CoA + H(2)O = CoA + acetate.,function:Hydrolyzes acetyl-CoA to acetate and CoA.,pathway:Carbohydrate metabolism; pyruvate metabolism.,similarity:Contains 1 START domain.,similarity:Contains 2 acyl coenzyme A hydrolase domains.,subunit:Homodimer or homotetramer.,
- Subcellular Location:
- Cytoplasm, cytosol .
- Expression:
- Chondrosarcoma Lung Metastasis,Liver,
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- Antibody-FAQs
- Products Images
- Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Immunofluorescence analysis of A549 cells, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide.