AAK1 Polyclonal Antibody
- Catalog No.:YT0038
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat;Monkey
- Target:
- AAK1
- Gene Name:
- AAK1
- Protein Name:
- AP2-associated protein kinase 1
- Human Gene Id:
- 22848
- Human Swiss Prot No:
- Q2M2I8
- Mouse Gene Id:
- 269774
- Mouse Swiss Prot No:
- Q3UHJ0
- Rat Swiss Prot No:
- P0C1X8
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human AAK1. AA range:271-320
- Specificity:
- AAK1 Polyclonal Antibody detects endogenous levels of AAK1 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- AAK1;KIAA1048;AP2-associated protein kinase 1;Adaptor-associated kinase 1
- Observed Band(KD):
- 94kD
- Background:
- Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008],
- Function:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Stimulated by clathrin.,function:Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subcellular location:Active when found in clathrin-coated pits at the plasma membrane.,subunit:Interacts with alpha-adaptin and AP-2.,
- Subcellular Location:
- Cell membrane ; Peripheral membrane protein . Membrane, clathrin-coated pit . Cell junction, synapse, presynapse . Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells. .
- Expression:
- Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using AAK1 Polyclonal Antibody
- Western blot analysis of lysates from COS7 cells, using AAK1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).