• Target：
• 4E-BP1

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>ErbB signaling pathway;>>HIF-1 signaling pathway;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Cellular senescence;>>Insulin signaling pathway;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Herpes simplex virus 1 infection;>>Chemical carcinogenesis - receptor activation;>>Acute myeloid leukemia;>>Choline metabolism in cancer

• Gene Name：
• EIF4EBP1

• Protein Name：
• Eukaryotic translation initiation factor 4E-binding protein 1

• Human Gene Id：
• 1978

• Human Swiss Prot No：
• Q13541

• Mouse Gene Id：
• 13685

• Mouse Swiss Prot No：
• Q60876

• Rat Gene Id：
• 116636

• Rat Swiss Prot No：
• Q62622

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human 4E-BP1. AA range:13-62

• Specificity：
• 4E-BP1 Polyclonal Antibody detects endogenous levels of 4E-BP1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• EIF4EBP1;Eukaryotic translation initiation factor 4E-binding protein 1;4E-BP1;eIF4E-binding protein 1;Phosphorylated heat- and acid-stable protein regulated by insulin 1;PHAS-I

• Observed Band(KD)：
• 18kD

• Background：
• eukaryotic translation initiation factor 4E binding protein 1(EIF4EBP1) Homo sapiens This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008],

• Function：
• function:Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.,PTM:Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eIF4E-binding protein family.,subunit:Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.,

• Subcellular Location：
• nucleoplasm,cytoplasm,cytosol,protein complex,

• Expression：
• Colon,Epithelium,Lung,Placenta,Platelet,

RAPTOR promotes colorectal cancer proliferation by inducing mTORC1 and upregulating ribosome assembly factor URB1. Cancer Medicine Cancer Med-Us. 2020 Feb;9(4):1529-1543 WB Human 1:2000 RKO cell, SW480 cell, HCT116 cell,LoVo cell

Gao, Hai-na, et al. "Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway 亮氨酸和组氨酸通过 mTOR 信号通路调控奶牛腺上皮细胞中酪蛋白的合成." Journal of Zhejiang University-SCIENCE B 16.6 (2015): 560-572.

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