14-3-3 θ/τ Polyclonal Antibody

    • Catalog No.:YT0011
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • 14-3-3 θ/τ
      • Fields:
      • >>Cell cycle;>>Oocyte meiosis;>>PI3K-Akt signaling pathway;>>Hippo signaling pathway;>>Hepatitis C;>>Hepatitis B;>>Viral carcinogenesis
      • Gene Name:
      • YWHAQ
      • Protein Name:
      • 14-3-3 protein theta
      • Human Swiss Prot No:
      • P27348
      • Mouse Swiss Prot No:
      • P68254
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human 14-3-3 thet/tau. AA range:196-245
      • Specificity:
      • 14-3-3 θ/τ Polyclonal Antibody detects endogenous levels of 14-3-3 θ/τ protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • YWHAQ;14-3-3 protein theta;14-3-3 protein T-cell;14-3-3 protein tau;Protein HS1
      • Observed Band(KD):
      • 28kD
      • Background:
      • This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008],
      • Function:
      • function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,similarity:Belongs to the 14-3-3 family.,subcellular location:In neurons, axonally transported to the nerve terminals.,subunit:Homodimer. Interacts with PCTK1 (By similarity). Interacts with SSH1. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm.,tissue specificity:Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in i
      • Subcellular Location:
      • Cytoplasm. In neurons, axonally transported to the nerve terminals.
      • Expression:
      • Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
      • Products Images
      • Immunofluorescence analysis of MCF7 cell. 1,primary Antibody was diluted at 1:100(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody(catalog No: RS3611) was diluted at 1:500(room temperature, 50min).
      • Western Blot analysis of various cells using 14-3-3 θ/τ Polyclonal Antibody diluted at 1:500
      • Western blot analysis of lysates from HeLa cells, using 14-3-3 thet/tau Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).