14-3-3 ζ Polyclonal Antibody

    • Catalog No.:YT0007
    • Applications:WB;IHC;IP;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • 14-3-3 ζ
      • Fields:
      • >>Cell cycle;>>Oocyte meiosis;>>PI3K-Akt signaling pathway;>>Hippo signaling pathway;>>Hepatitis C;>>Hepatitis B;>>Viral carcinogenesis
      • Gene Name:
      • YWHAZ
      • Protein Name:
      • 14-3-3 protein zeta/delta
      • Human Gene Id:
      • 7534
      • Human Swiss Prot No:
      • P63104
      • Mouse Swiss Prot No:
      • P63101
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta. AA range:24-73
      • Specificity:
      • 14-3-3 ζ Polyclonal Antibody detects endogenous levels of 14-3-3 ζ protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • YWHAZ;14-3-3 protein zeta/delta;Protein kinase C inhibitor protein 1;KCIP-1
      • Observed Band(KD):
      • 28kD
      • Background:
      • This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],
      • Function:
      • caution:Was originally (PubMed:1577711) thought to have phospholipase A2 activity.,function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,PTM:The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.,similarity:Belongs to the 14-3-3 family.,subcellular location:Located to
      • Subcellular Location:
      • Cytoplasm . Melanosome . Located to stage I to stage IV melanosomes.
      • Expression:
      • B-cell lymphoma,Bone marrow
      • Products Images
      • Western Blot analysis of various cells using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of COLO205 cells using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of 293T HELA using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunofluorescence analysis of HeLa cells, using 14-3-3 zeta Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 zeta Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from 293 cells, treated with Forskolin 40nM 30', using 14-3-3 zeta Antibody. The lane on the right is blocked with the synthesized peptide.