• Target：
• PI 3 Kinase Class 3

• Fields：
• >>Inositol phosphate metabolism;>>Metabolic pathways;>>Phosphatidylinositol signaling system;>>Autophagy - other;>>Autophagy - animal;>>Phagosome;>>Apelin signaling pathway;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Salmonella infection;>>Tuberculosis;>>Kaposi sarcoma-associated herpesvirus infection

• Gene Name：
• PIK3C3 VPS34

• Protein Name：
• PI3 Kinase Class III (Ser249)

• Human Gene Id：
• 5289

• Human Swiss Prot No：
• Q8NEB9

• Mouse Gene Id：
• 225326

• Mouse Swiss Prot No：
• Q6PF93

• Rat Gene Id：
• 65052

• Rat Swiss Prot No：
• O88763

• Immunogen：
• Synthesized phosho peptide around human PI3 Kinase Class III (Ser249)

• Specificity：
• This antibody detects endogenous levels of Human PI3 Kinase Class III (phospho-Ser249)

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000

• Purification：
• The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Phosphatidylinositol 3-kinase catalytic subunit type 3 (PI3-kinase type 3) (PI3K type 3) (PtdIns-3-kinase type 3) (EC 2.7.1.137) (Phosphatidylinositol 3-kinase p100 subunit) (Phosphoinositide-3-kinase class 3) (hVps34)

• Observed Band(KD)：
• 100kD

• Function：
• catalytic activity:ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.,cofactor:Manganese.,function:Catalytic subunit of the PI3K complex. Involved in the transport of lysosomal enzyme precursors to lysosomes.,similarity:Belongs to the PI3/PI4-kinase family.,similarity:Contains 1 PI3K/PI4K domain.,subunit:Probably forms a complex with AMBRA1 and BECN1 (By similarity). Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4.,tissue specificity:Ubiquitously expressed, with a highest expression in skeletal muscle.,

• Subcellular Location：
• Midbody . Late endosome . Cytoplasmic vesicle, autophagosome . As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (PubMed:14617358). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity). .

• Expression：
• Ubiquitously expressed, with a highest expression in skeletal muscle.

Zhongfeng Capsules protects against cerebral ischemia–reperfusion injury via mediating the phosphoinositide 3-kinase/Akt and toll-like receptor 4/nuclear factor kappa B signaling pathways by regulating neuronal apoptosis and inflammation WB Rat 1:1000 brain tissue/

Proteomic Study on the Reproductive Toxicity of Tripterygium Glycosides in Rats WB Rat testis tissue /

Induction of PI3K/Akt-Mediated Apoptosis in Osteoclasts Is a Key Approach for Buxue Tongluo Pills to Treat Osteonecrosis of the Femoral Head.. Frontiers in Pharmacology Front Pharmacol. 2021 Nov;12:729909-729909 WB Mouse 1:500，1:1000 RAW 264.7 cell

Fucoidan inhibits lymphangiogenesis by downregulating the expression of VEGFR3 and PROX1 in human lymphatic endothelial cells. Oncotarget Oncotarget. 2016 Jun 21; 7(25): 38025–38035 WB Human HLECs

An Integrated Approach Based on Network Pharmacology Combined with Experimental Verification Reveals AMPK/PI3K/Akt Signaling is an Important Way for the Anti-Type 2 Diabetic Activity of Silkworm Excrement. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy Diabet Metab Synd Ob. 2021 Feb;14:601-616 WB Human 1:1000 HepG2 cell

Aaptamine attenuates the proliferation and progression of non-small cell lung carcinoma. PHARMACEUTICAL BIOLOGY Pharm Biol. 2020;58(1):1044-1054 WB Human A549 cell，H1299 cell

The negative charge of the 343 site is essential for maintaining physiological functions of CXCR4. BMC Molecular and Cell Biology Bmc Mol Cell Biol. 2021 Dec;22(1):1-10 WB Human 1:1000 HeLa cell

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