• Target：
• DAPK2

• Fields：
• >>Autophagy - animal;>>Pathways in cancer;>>Bladder cancer

• Gene Name：
• DAPK2

• Protein Name：
• Death-associated protein kinase 2

• Human Gene Id：
• 23604

• Human Swiss Prot No：
• Q9UIK4

• Mouse Gene Id：
• 13143

• Mouse Swiss Prot No：
• Q8VDF3

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human DAPK2 around the phosphorylation site of Ser318. AA range:284-333

• Specificity：
• Phospho-DAPK2 (S318) Polyclonal Antibody detects endogenous levels of DAPK2 protein only when phosphorylated at S318.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• DAPK2;Death-associated protein kinase 2;DAP kinase 2;DAP-kinase-related protein 1;DRP-1

• Molecular Weight(Da)：
• 43kD

• Background：
• This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-318.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer. Homodimerization is required for apoptotic function and is inhibited by autophosphorylation at Ser-318.,tissue specificity:Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.,

• Subcellular Location：
• Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.

• Expression：
• Expressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle.

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