• Target：
• Myt 1

• Fields：
• >>Cell cycle;>>Oocyte meiosis;>>Progesterone-mediated oocyte maturation

• Gene Name：
• PKMYT1

• Protein Name：
• Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase

• Human Gene Id：
• 9088

• Human Swiss Prot No：
• Q99640

• Mouse Swiss Prot No：
• Q9ESG9

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human MYT1 around the phosphorylation site of Ser83. AA range:49-98

• Specificity：
• Phospho-Myt 1 (S83) Polyclonal Antibody detects endogenous levels of Myt 1 protein only when phosphorylated at S83.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PKMYT1;MYT1;Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase;Myt1 kinase

• Molecular Weight(Da)：
• 55kD

• Background：
• This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.,enzyme regulation:Negatively regulated by hyperphosphorylation during mitosis. The hyperphosphorylated form does not associate with CCNB1-CDC2 complexes. The PLK1 protein kinase may be required for mitotic phosphorylation.,function:Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the cdc2 kinase specifically when cdc2 is complexed to cyclins. Mediates phosphorylation of cdc2 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of cdc2 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be ind

• Subcellular Location：
• Endoplasmic reticulum membrane ; Peripheral membrane protein . Golgi apparatus membrane ; Peripheral membrane protein .

• Expression：
• Brain,Epithelium,PCR rescued clones,

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