• Target：
• p40-phox

• Fields：
• >>Phagosome;>>Osteoclast differentiation;>>Neutrophil extracellular trap formation;>>Leukocyte transendothelial migration;>>Prion disease;>>Leishmaniasis;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis

• Gene Name：
• NCF4

• Protein Name：
• Neutrophil cytosol factor 4

• Human Gene Id：
• 4689

• Human Swiss Prot No：
• Q15080

• Mouse Gene Id：
• 17972

• Mouse Swiss Prot No：
• P97369

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human p40 phox around the phosphorylation site of Thr154. AA range:120-169

• Specificity：
• Phospho-p40-phox (T154) Polyclonal Antibody detects endogenous levels of p40-phox protein only when phosphorylated at T154.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NCF4;SH3PXD4;Neutrophil cytosol factor 4;NCF-4;Neutrophil NADPH oxidase factor 4;SH3 and PX domain-containing protein 4;p40-phox;p40phox

• Molecular Weight(Da)：
• 39kD

• Background：
• The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding d

• Function：
• function:Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 1 SH3 domain.,subunit:p40-PHOX associates primarily with p67-PHOX to form a complex with p47-PHOX.,tissue specificity:Expression is restricted to hematopoietic cells.,

• Subcellular Location：
• Cytoplasm, cytosol . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Membrane ; Peripheral membrane protein .

• Expression：
• Expression is restricted to hematopoietic cells.

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