Net (phospho Ser357) Polyclonal Antibody

    • Catalog No.:YP0920
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Monkey
      • Target:
      • Net
      • Gene Name:
      • ELK3
      • Protein Name:
      • ETS domain-containing protein Elk-3
      • Human Gene Id:
      • 2004
      • Human Swiss Prot No:
      • P41970
      • Mouse Swiss Prot No:
      • P41971
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Elk3 around the phosphorylation site of Ser357. AA range:323-372
      • Specificity:
      • Phospho-Net (S357) Polyclonal Antibody detects endogenous levels of Net protein only when phosphorylated at S357.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ELK3;NET;SAP2;ETS domain-containing protein Elk-3;ETS-related protein ERP;ETS-related protein NET;Serum response factor accessory protein 2;SAP-2;SRF accessory protein 2
      • Observed Band(KD):
      • 38kD
      • Background:
      • This gene encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015],
      • Function:
      • function:May be a negative regulator of transcription, but can activate transcription when coexpressed with Ras, Src or Mos. Forms a ternary complex with the serum response factor and the ETS and SRF motifs of the Fos serum response element.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Interacts with CTBP1.,
      • Subcellular Location:
      • Nucleus.
      • Expression:
      • Brain,Epithelium,Placenta,
      • Products Images
      • Western Blot analysis of COLO cells using Phospho-Net (S357) Polyclonal Antibody
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Elk3 (Phospho-Ser357) Antibody
      • Immunofluorescence analysis of HeLa cells, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using Elk3 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.