Calmodulin-1 (phospho Thr80/S82) Polyclonal Antibody
- Catalog No.：YP0912
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Mouse;Rat
- Target：
- Calmodulin-1
- Gene Name：
- CALM1
- Protein Name：
- Calmodulin-1
- Human Gene Id：
- 801/805/808
- Human Swiss Prot No：
- P0DP23
- Mouse Gene Id：
- 12313
- Mouse Swiss Prot No：
- P0DP26
- Rat Gene Id：
- 24242
- Rat Swiss Prot No：
- P0DP29
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human Calmodulin-1 around the phosphorylation site of Thr79 and Ser81. AA range:46-95
- Specificity：
- Phospho-Calmodulin (T80/S82) Polyclonal Antibody detects endogenous levels of Calmodulin protein only when phosphorylated at T80/S82.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- CALM1;CALM;CAM;CAM1;CALM2;CAM2;CAMB;CALM3;CALML2;CAM3;CAMC;CAMIII;Calmodulin;CaM
- Molecular Weight(Da)：
- 17kD
- Background：
- This gene encodes a member of the EF-hand calcium-binding protein family. It is one of three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009],
- Function：
- function:Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.,miscellaneous:This protein has four functional calcium-binding sites.,PTM:Phosphorylation results in a decreased activity.,PTM:Ubiquitination results in a strongly decreased activity.,similarity:Belongs to the calmodulin family.,similarity:Contains 4 EF-hand domains.,subcellular location:Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.,subunit:Interacts with MYO1C (By similarity). Interacts with CEP97, CEP110, TTN/titin and SRY.,
- Subcellular Location：
- spindle pole,extracellular region,nucleus,nucleoplasm,cytoplasm,centrosome,cytosol,spindle microtubule,plasma membrane,voltage-gated potassium channel complex,sarcomere,growth cone,vesicle,calcium channel complex,G
- Expression：
- Blood,Brain,Cajal-Retzius cell,Fetal brain cortex,Lung,Lymph,Lymphoma,Muscle,Osteosarcoma,P
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
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- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
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- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using Phospho-Calmodulin (T80/S82) Polyclonal Antibody
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Calmodulin (Phospho-Thr79+Ser81) Antibody
- Immunofluorescence analysis of HepG2 cells, using Calmodulin (Phospho-Thr79+Ser81) Antibody. The picture on the right is blocked with the phospho peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain, using Calmodulin (Phospho-Thr79+Ser81) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from Jurkat cells treated with Insulin 0.01U/ml 15', using Calmodulin (Phospho-Thr79+Ser81) Antibody. The lane on the right is blocked with the phospho peptide.
