p70 S6 kinase α (phospho Ser418) Polyclonal Antibody

    • Catalog No.:YP0886
    • Applications:WB,IHC-p,IF/ICC,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • RPS6KB1
      • Protein Name:
      • Ribosomal protein S6 kinase beta-1
      • Human Gene Id:
      • 6198
      • Human Swiss Prot No:
      • P23443
      • Mouse Swiss Prot No:
      • Q8BSK8
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Ser418. AA range:384-433
      • Specificity:
      • Phospho-p70 S6 kinase α (S418) Polyclonal Antibody detects endogenous levels of p70 S6 kinase α protein only when phosphorylated at S418.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • RPS6KB1; STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinas
      • MolecularWeight(Da):
      • 59140
      • Observed Band(KD):
      • 60
      • Background:
      • ribosomal protein S6 kinase B1(RPS6KB1) Homo sapiens This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activation by serine/threonine phosphorylation and protein kinase C, inactivated by type 2A phosphatase.,function:Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with PPP1R9A/neurabin-1.,tissue specificity:Widely expressed.,
      • Subcellular Location:
      • nucleus,nucleoplasm,cytoplasm,mitochondrion,mitochondrial outer membrane,cytosol,cell surface,cell junction,neuron projection,synapse,perinuclear region of cytoplasm,
      • Expression:
      • Epithelium,Eye,Testis,
      • Products Images
      • Western blot analysis of HT-29 serum 20% 15' using p-p70 S6 kinase α (S418) antibody. Antibody was diluted at 1:1000
      • The picture was kindly provided by our customer,antibody was diluted at 1:500
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p70 S6 Kinase (Phospho-Ser418) Antibody
      • Immunofluorescence analysis of COS7 cells, using p70 S6 Kinase (Phospho-Ser418) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Ser418) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HT29 cells treated with serum 20% 15', using p70 S6 Kinase (Phospho-Ser418) Antibody. The lane on the right is blocked with the phospho peptide.