EF-2 (phospho Thr56) Polyclonal Antibody
- Catalog No.：YP0870
- Applications：WB,IHC-p,IF,ELISA
- Reactivity：Human,Mouse,Rat
- Description
- Gene Name：
- EEF2
- Protein Name：
- Elongation factor 2
- Human Gene Id：
- 1938
- Human Swiss Prot No：
- P13639
- Mouse Gene Id：
- 13629
- Mouse Swiss Prot No：
- P58252
- Rat Gene Id：
- 29565
- Rat Swiss Prot No：
- P05197
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human eEF2 around the phosphorylation site of Thr56. AA range:31-80
- Specificity：
- Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Rabbit
- Dilution：
- Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -20°C/1 year
- Other Name：
- EEF2; EF2; Elongation factor 2; EF-2
- MolecularWeight(Da)：
- 95207
- Observed Band(KD)：
- 100
- Background：
- EEF2 encodes a member of the GTP-binding translation elongation factor family. Elongation factor 2 is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
- Function：
- translation, translational elongation,
- Products Images
- Western Blot analysis of SKOV3 cells using Phospho-EF-2 (T56) Polyclonal Antibody diluted at 1：2000
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eEF2 (Phospho-Thr56) Antibody
- Immunofluorescence analysis of HUVEC cells, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from NIH/3T3 cells treated with Serum 10% 30', using eEF2 (Phospho-Thr56) Antibody. The lane on the right is blocked with the phospho peptide.
