EF-2 (phospho Thr56) Polyclonal Antibody
- Catalog No.:YP0870
- Applications:WB,IHC-p,IF/ICC,ELISA
- Reactivity:Human,Mouse,Rat
- Gene Name:
- EEF2
- Protein Name:
- Elongation factor 2
- Human Gene Id:
- 1938
- Human Swiss Prot No:
- P13639
- Mouse Gene Id:
- 13629
- Mouse Swiss Prot No:
- P58252
- Rat Gene Id:
- 29565
- Rat Swiss Prot No:
- P05197
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human eEF2 around the phosphorylation site of Thr56. AA range:31-80
- Specificity:
- Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Rabbit
- Dilution:
- Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -20°C/1 year
- Other Name:
- EEF2; EF2; Elongation factor 2; EF-2
- MolecularWeight(Da):
- 95207
- Observed Band(KD):
- 100
- Background:
- eukaryotic translation elongation factor 2(EEF2) Homo sapiens This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008],
- Function:
- function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.,PTM:Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.,PTM:Phosphorylation by EF-2 kinase completely inactivates EF-2.,similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.,
- Subcellular Location:
- nucleus,cytoplasm,cytosol,plasma membrane,cell-cell adherens junction,membrane,aggresome,intracellular ribonucleoprotein complex,extracellular matrix,polysomal ribosome,membrane raft,extracellular exosome
- Expression:
- Brain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of SKOV3 cells using Phospho-EF-2 (T56) Polyclonal Antibody diluted at 1:2000
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eEF2 (Phospho-Thr56) Antibody
- Immunofluorescence analysis of HUVEC cells, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from NIH/3T3 cells treated with Serum 10% 30', using eEF2 (Phospho-Thr56) Antibody. The lane on the right is blocked with the phospho peptide.