EF-2 (phospho Thr56) Polyclonal Antibody

    • Catalog No.:YP0870
    • Applications:WB,IHC-p,IF,ELISA
    • Reactivity:Human,Mouse,Rat
    • Description
    • Gene Name:
    • EEF2
    • Protein Name:
    • Elongation factor 2
    • Human Gene Id:
    • 1938
    • Human Swiss Prot No:
    • P13639
    • Mouse Swiss Prot No:
    • P58252
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from human eEF2 around the phosphorylation site of Thr56. AA range:31-80
    • Specificity:
    • Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • EEF2; EF2; Elongation factor 2; EF-2
    • MolecularWeight(Da):
    • 95207
    • Observed Band(KD):
    • 100
    • Background:
    • EEF2 encodes a member of the GTP-binding translation elongation factor family. Elongation factor 2 is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
    • Function:
    • translation, translational elongation,
    • Products Images
    • Western Blot analysis of SKOV3 cells using Phospho-EF-2 (T56) Polyclonal Antibody diluted at 1:2000
    • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eEF2 (Phospho-Thr56) Antibody
    • Immunofluorescence analysis of HUVEC cells, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.
    • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.
    • Western blot analysis of lysates from NIH/3T3 cells treated with Serum 10% 30', using eEF2 (Phospho-Thr56) Antibody. The lane on the right is blocked with the phospho peptide.