• Gene Name：
• EEF2

• Protein Name：
• Elongation factor 2

• Human Gene Id：
• 1938

• Human Swiss Prot No：
• P13639

• Mouse Gene Id：
• 13629

• Mouse Swiss Prot No：
• P58252

• Rat Gene Id：
• 29565

• Rat Swiss Prot No：
• P05197

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human eEF2 around the phosphorylation site of Thr56. AA range:31-80

• Specificity：
• Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Rabbit

• Dilution：
• Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -20°C/1 year

• Other Name：
• EEF2; EF2; Elongation factor 2; EF-2

• MolecularWeight(Da)：
• 95207

• Observed Band(KD)：
• 100

• Background：
• eukaryotic translation elongation factor 2(EEF2) Homo sapiens This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008],

• Function：
• function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.,PTM:Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.,PTM:Phosphorylation by EF-2 kinase completely inactivates EF-2.,similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.,

• Subcellular Location：
• nucleus,cytoplasm,cytosol,plasma membrane,cell-cell adherens junction,membrane,aggresome,intracellular ribonucleoprotein complex,extracellular matrix,polysomal ribosome,membrane raft,extracellular exosome

• Expression：
• Brain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph

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